Functional characteristic and differential expression of myostatin in Chlamys nobilis
文献类型: 外文期刊
第一作者: Zhu, Kecheng
作者: Zhu, Kecheng;Guo, Huayang;Zhang, Nan;Li, Yundong;Jiang, Shigui;Zhang, Dianchang
作者机构:
关键词: Chlamys nobilis;myostatin;phylogenetic trees;gene expression
期刊名称:JOURNAL OF APPLIED ANIMAL RESEARCH ( 影响因子:1.63; 五年影响因子:1.727 )
ISSN: 0971-2119
年卷期: 2017 年 46 卷 1 期
页码:
收录情况: SCI
摘要: Myostatin (MSTN) was a conserved negative regulatory protein of muscle growth and development in numerous species. In this study, the MSTN gene was cloned and characterized from a noble scallop, Chlamys nobilis, and it was named CN-MSTN. To investigate the molecular characterization of MSTN and its gene expression profile of spatio-temporal, we isolated the MSTN cDNA sequence in C.nobilis and analysed expression patterns using quantitative real-time PCR. CN-MSTN cDNA contained a 1,374bp open reading frame that encoded a 458 amino acids. Furthermore, the result of 3D model indicated that CN-MSTN mature peptide was similar to that of Chlamys farreri. Additionally, based on both the nucleotide and amino acid datasets, phylogenetic trees indicated that C.nobilis has the highest similarity with homologues of C.farreri. The expression patterns of different stages showed that MSTN expression was markedly higher in the gastrulae period. Additionally, remarkable expression of MSTN was observed in adductor muscle than with other tissues. Our data would provide expression and phylogenetic information of this economical important sea food, noble scallop.
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