Construction of a high-density linkage map and mapping of sex determination and growth-related loci in the mandarin fish (Siniperca chuatsi)

文献类型： 外文期刊

第一作者： Sun, Chengfei

作者： Sun, Chengfei;Ye, Xing;Dong, Junjian;Zeng, Qingkai;Chen, Zhihang;Tian, Yuanyuan;Lu, Maixin;Niu, Yongchaox;Hu, Wushu;Zeng, Qingkai;Chen, Zhihang;Tian, Yuanyuan;Zhang, Jin

作者机构：

关键词： Siniperca chuatsi;Linkage map;Sex determination;Growth-related trait;Quantitative trait locus

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2017 年 18 卷

页码：

收录情况： SCI

摘要： Background: The mandarin fish (Siniperca chuatsi) is an important and widely cultured fish in China. However, the lack of selective breeding of mandarin fish in previous decades has resulted in a decline in the growth rate of pond-cultured fish, a shortened period of sexual maturity, and reduced disease resistance; these issues seriously affect the quality and safety of the fish products. Therefore, it is necessary to establish a selective breeding program for the mandarin fish to improve the economical traits of the fish and to sustain the development of the mandarin fish industry. Results: We constructed a high-density linkage map for it based on double digest restriction site associated DNA sequencing (ddRAD-Sequencing). This map contained 3283 dimorphic single nucleotide polymorphism markers and 24 linkage groups (LGs). The total map-length was 1972.01 cM, with an average interlocus distance of 0.61 cM. One significant quantitative trait locus (QTL) for sex determination trait was detected on LG23, which was supported by five markers, clustered between 60.27 and 68.71 cM. The highest logarithm of odds value (17.73) was located at 60.27 cM, near the marker r1_73194, accounting for 53.3% of the phenotypic variance. Genotypes of all the male fish on r1_33008 were homozygous, whereas those of all females were heterozygous. Thus, LG23 was considered a sex-related linkage group. Eleven significant QTLs, for three growth traits, at two growth stages and the increased values were distributed on four LGs; their contributions to the phenotypic variation were quite low (12.4-17.2%), suggesting that multiple genes affected the growth traits. Conclusion: This high-resolution genetic map provides a valuable resource for fine-mapping of important traits and for identification of sex-related markers that should facilitate breeding of all-female mandarin fish for aquaculture and mechanistic studies on sex determination.

分类号：

  • 相关文献

[1]CONSTRUCTION AND CHARACTERIZATION OF A TENTATIVE AMPLIFIED FRAGMENT LENGTH POLYMORPHISM-SIMPLE SEQUENCE REPEAT LINKAGE MAP OF LAMINARIA (LAMINARIALES, PHAEOPHYTA). Yang, Guanpin,Sun, Ying,Shi, Yuanyuan,Zhang, Linan,Guo, Shanshan,Li, Bingjun,Li, Xiaojie,Li, Zhiling,Cong, Yizhou,Zhao, Yushan,Wang, Wenquan.

[2]Identification of quantitative trait loci for growth-related traits in the swimming crab Portunus trituberculatus. Liu, Lei,Li, Jian,Liu, Ping,Zhao, Fazhen,Gao, Baoquan,Du, Ying,Liu, Lei,Du, Ying.

[3]Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi. Sun, C. F.,Ye, X.,Tian, Y. Y.,Dong, J. J.. 2015

[4]The pathogenicity and biological features of Santee-Cooper Ranaviruses isolated from Chinese perch and snakehead fish. Fu, Xiaozhe,Lin, Qiang,Liu, Lihui,Liang, Hongru,Huang, Zhibin,Li, Ningqiu. 2017

[5]Protein encoded by ORF093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in Chinese perch Siniperca chuatsi. Li, Ningqiu,Fu, Xiaozhe,Guo, Huizhi,Lin, Qiang,Liu, Lihui,Zhang, Defeng,Fang, Xiang,Wu, Shuqin,Li, Ningqiu,Fu, Xiaozhe,Guo, Huizhi,Lin, Qiang,Liu, Lihui,Zhang, Defeng,Fang, Xiang,Wu, Shuqin.

[6]Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation. Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,Xu, Z.,Yao, W. J.,Nie, P.,Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,Xu, Z.,Yao, W. J.,Nie, P.,Tian, J. Y..

[7]Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi. Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei.

[8]Transcriptomic analysis of Mandarin fish brain cells infected with infectious spleen and kidney necrosis virus with an emphasis on retinoic acid-inducible gene 1-like receptors and apoptosis pathways. Hu, Xianqin,Dong, Xingxing,Zhao, Lijuan,Lan, Jiangfeng,Ji, Wei,Lin, Li,Fu, Xiaozhe,Li, Ningqiu,Wu, Shuqin,Hu, Xianqin,Fu, Xiaozhe,Li, Ningqiu,Wu, Shuqin,Lin, Li,Hu, Xianqin,Zhou, Weidong,Ai, Taoshan,Lin, Li.

[9]Early protein ORF086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish Siniperca chuatsi. Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Liu, Lihui,Huang, Zhibin,Wu, Shuqin,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Liu, Lihui,Wu, Shuqin.

[10]Mandarin fish p53: Genomic structure, alternatively spliced variant and its mRNA expression after virus challenge. Guo, Huizhi,Su, Jianguo,Guo, Huizhi,Fu, Xiaozhe,Lin, Qiang,Liu, Lihui,Liang, Hongru,Huang, Zhibin,Li, Ningqiu.

[11]Molecular characterization and expression pattern of tumor suppressor protein p53 in mandarin fish, Siniperca chuatsi following virus challenge. Guo, Huizhi,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Liu, Lihui,Wu, Shuqin,Guo, Huizhi,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Liu, Lihui,Wu, Shuqin.

[12]Polymorphism in Growth Hormone Gene and its Association with Growth Traits in Siniperca chuatsi. Wang, Haifang,Xu, Peng,Li, Guifeng,Wang, Haifang,Xu, Peng,Li, Guifeng,Wang, Haifang,Xu, Peng,Li, Guifeng,Wang, Haifang,Sun, Jijia,Wang, Pengfei,Lu, Xue,Gu, Yongming. 2016

[13]Purification and characterisation of trypsins from the pyloric caeca of mandarin fish (Siniperca chuatsi). Lu, Bao-Ju,Cai, Qiu-Feng,Su, Wen-Jin,Cao, Min-Jie,Zhou, Li-Gen,Hara, Kenji,Maeda, Asami.

[14]Protective immunity against infectious spleen and kidney necrosis virus induced by immunization with DNA plasmid containing mcp gene in Chinese perch Siniperca chuatsi. Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Zhang, Defeng,Liu, Lihui,Wu, Shuqin,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Zhang, Defeng,Liu, Lihui,Wu, Shuqin.

[15]Sex-biased miRNAs of yellow catfish (Pelteobagrus fulvidraco) and their potential role in reproductive development. Wang, Pingping,Wang, Le,Yang, Jian,Luan, Peixian,Zhang, Xiaofeng,Kuang, Youyi,Sun, Xiaowen. 2018

[16]Key Signaling Events for Committing Mouse Pluripotent Stem Cells to the Germline Fate. Wang, Jian-Qi,Cao, Wen-Guang.

[17]Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties. Liu, Yating,Liu, Yating,Xie, Wen,Yang, Xin,Guo, Litao,Wang, Shaoli,Wu, Qingjun,Yang, Zezhong,Zhang, Youjun,Zhou, Xuguo.

[18]Sex-lethal gene of the Chinese mitten crab Eriocheir sinensis: cDNA cloning, induction by eyestalk ablation, and expression of two splice variants in males and females. Shen, Huaishun,Hu, Yacheng,Zhou, Xin,Shen, Huaishun,Hu, Yacheng.

[19]Establishment and characterization of fibroblast cell line of the Songliao Black pig. Liu, Xuedong,Li, Lin-Feng,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Xuedong,Li, Lin-Feng.

[20]Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense. Zhang, Y. P.,Zhang, W. Y.,Fu, H. T.,Zhang, Y. P.,Qiao, H.,Sun, S. M.,Jiang, S. F.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.. 2013

作者其他论文 更多>>

微信小程序