Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics
文献类型: 外文期刊
第一作者: Liu, Guangjin
作者: Liu, Guangjin;Zhang, Wei;Liu, Yongjie;Yao, Huochun;Lu, Chengping;Xu, Pao
作者机构:
关键词: Streptococcus agalactiae;Piscine;Pre-absorbed immunoproteomic method (PAIM);Serine-rich repeat protein (Srr);Zebrafish
期刊名称:BMC VETERINARY RESEARCH ( 影响因子:2.741; 五年影响因子:2.955 )
ISSN: 1746-6148
年卷期: 2014 年 10 卷
页码:
收录情况: SCI
摘要: Background: Since 2009, large-scale Streptococcus agalactiae infections have broken out in cultured tilapia farms in China, resulting in considerable economic losses. Screening of the surface proteins is required to identify virulence factors or protective antigens involved in piscine S. agalactiae infections in tilapia. Pre-absorbed immunoproteomics method (PAIM) is a useful method previously established in our laboratory for identifying bacterial surface proteins. Results: A serine-rich repeat protein family 1 (Srr-1), designated XF, was identified by PAIM in piscine S. agalactiae isolate GD201008-001. To investigate the role of XF in the pathogenesis of piscine S. agalactiae, an isogenic xf mutant strain (Delta xf) and a complemented strain (C Delta xf) were successfully constructed. The Delta xf mutant and C Delta xf showed no significant differences in growth characteristics and adherence to HEp-2 cells compared with the wild-type strain. However the 50% lethal dose of Delta xf was increased (4-fold) compared with that of the parental strain in a zebrafish infection model. Conclusions: The findings demonstrated that XF is a virulence-related, highly immunoreactive surface protein and is involved in the pathogenicity of S. agalactiae infections in fish.
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