Cloning and Characterization of tesk1, a Novel Spermatogenesis-Related Gene, in the Tongue Sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Meng, Liang

作者： Meng, Liang;Meng, Liang;Zhu, Ying;Zhang, Ning;Liu, Wanjun;Liu, Yang;Shao, Changwei;Wang, Na;Chen, Songlin

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 10 期

页码：

收录情况： SCI

摘要： Testis-specific protein kinase 1 (Tesk1) is a serine/threonine kinase with unique structural features. In the present study, we cloned and characterized the tesk1 gene of tongue sole, Cynoglossus semilaevis. The full-length tesk1 cDNA consists of 1,672 nucleotides, encoding a 331 amino acid polypeptide with a characteristic structure composed of an N-terminal kinase domain and a C-terminal proline-rich domain. The tesk1 genomic sequence contains eight exons and seven introns. Real-time quantitative PCR revealed that tesk1 mRNA is expressed predominantly in the testis, though the level of expression varied throughout development. We used in situ hybridization to show that tesk1 mRNA is expressed in the spermatids of males and pseudo-males, but not in triploid males. Our results suggest that tongue sole Tesk1 may play a role in spermatogenesis.

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