EFFECTS OF CRYOPRESERVATION ON MOTILITY CHARACTERISTICS AND ENZYME ACTIVITY OF SPERM IN A CHINESE FISH, Nibea albiflora
文献类型: 外文期刊
第一作者: Huang, Xiaorong
作者: Huang, Xiaorong;Zhuang, Ping;Zhang, Longzhen;Zhao, Feng;Liu, Jianyi;Feng, Guangpeng;Zhang, Tao
作者机构:
关键词: Cryopreservation;Nibea albiflora;sperm motility;enzyme activity;fertilizing capacity
期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )
ISSN: 0143-2044
年卷期: 2014 年 35 卷 4 期
页码:
收录情况: SCI
摘要: BACKGROUND: Growing evidence suggests that among the causes which deteriorate qualitative and functional characteristics of sperm after freezing and thawing, there are those linked to decrease of sperm motility and release of various enzymes in the cells and seminal plasma. OBJECTIVE: In the present study, the motility, fertilization and enzyme activity of sperm were analyzed after cryopreservation. MATERIALS AND TMETHODS: Computer-assisted sperm motility analysis (CASA) was used to evaluate the effect of cryopreservation on sperm motility of Nibea albiflora. RESULTS: The activities of total adenosine triphosphatase (ATPase), creatine kinase (CK), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) in fresh and frozen seminal plasma and spermatozoa were measured respectively. Cryopreservation led to a decline in the percentage of motile sperm, moreover, other parameters of sperm motion, curvilinear and straight line velocities, linearity were changed observably (p<0.05), the fertilizing capacity of post-thaw sperm was lower than that of the fresh sperm significantly. After cryopreservation, the activities of total ATPase, CK, SDH, LDH, SOD, CAT and GSH-Px increased in seminal plasma and decreased in spermatozoa respectively, but GR activity varied contrarily, GR activity dropped in seminal plasma and increased in spermatozoa. CONCLUSION: Cryopreservation had significant effects on the motility characteristics, fertilization ability and enzyme activity of the sperm of Nibea albiflora.
分类号:
- 相关文献
作者其他论文 更多>>
-
Versatile plant genome engineering using anti-CRISPR-Cas12a systems
作者:He, Yao;Xu, Tang;Zheng, Xuelian;Zhang, Yong;He, Yao;Liu, Shishi;Pu, Dongkai;Zhong, Zhaohui;Ren, Qiurong;Dong, Chuan;Wang, Yawei;Wang, Danning;Zhang, Yong;Liu, Shishi;Chen, Long;Zhang, Tao;Chen, Long;Zhang, Tao;Guo, Fengbiao;Guo, Fengbiao;Guo, Fengbiao;Qi, Yiping;Qi, Yiping
关键词:anti-CRISPR; Cas12a; AcrVA1; off-target effects; fine-tuning genome editing; inducible and tissue specific genome editing; CRISPRa; synthetic logic circuit
-
Genetic diversity and population structure in five Inner Mongolia cashmere goat populations using whole-genome genotyping
作者:Zhang, Tao;Wang, Zhiying;Li, Yaming;Zhou, Bohan;Liu, Yifan;Wang, Ruijun;Lv, Qi;Zhang, Yanjun;Su, Rui;Zhang, Tao;Wang, Zhiying;Li, Yaming;Zhou, Bohan;Liu, Yifan;Li, Jinquan;Wang, Ruijun;Lv, Qi;Zhang, Yanjun;Su, Rui;Wang, Zhiying;Li, Yaming;Zhou, Bohan;Liu, Yifan;Li, Jinquan;Wang, Ruijun;Lv, Qi;Zhang, Yanjun;Su, Rui;Li, Chun
关键词:Cashmere Goat; Genetic Diversity; Inner Mongolia Autonomous Region; Population Structure
-
Gonadal Transcriptome Sequencing Analysis Reveals the Candidate Sex-Related Genes and Signaling Pathways in the East Asian Common Octopus, Octopus sinensis
作者:Li, Fenghui;Chen, Siqing;Pan, Luying;Liu, Changlin;Bian, Li;Li, Fenghui;Chen, Siqing;Pan, Luying;Liu, Changlin;Bian, Li;Zhang, Tao
关键词:Octopus sinensis; gonadal transcriptome sequence; gene-expression profile; sex-related genes and pathways
-
Functional analysis of the CaPIPLC5 gene in the regulation of the fertility restoration in pepper
作者:Ma, Yan;Zhang, Tao;Wang, Yuhang;Wang, Lina;Kong, Weifu;Zhang, Gaoyuan;Wei, Bingqiang;Wei, Ming;Duan, Panpan
关键词:Cytoplasmic male sterility; Fertility restoration; Pepper; Phospholipase C; Transcription factor
-
Transcriptomics and metabolomics of the molecular mechanisms of ginseng's response to the continuous cropping obstacle
作者:Shen, Yanlong;Zhan, Yu;Zhang, Tao;Li, Qiong;Wang, Enpeng;Chen, Changbao;Shen, Yanlong;Zhang, Hao
关键词:Panax ginseng; Metabolome; Transcriptome; Environmental stress; Continuous cropping obstacle
-
Flavonoid-based yellow dye extract from safflower (Carthamus tinctorius L.) combined with chitosan for anti-bacterial and ultraviolet-protective functionalisation of silk
作者:Do, Khai Ly;Zhao, Feng;Su, Miao;Do, Khai Ly;Zhao, Feng;Su, Miao;Mushtaq, Asim;Yousaf, Muhammad;Ahsan, Taswar;Su, Miao
关键词:
-
N123I mutation in the ALV-J receptor-binding domain region enhances viral replication ability by increasing the binding affinity with chNHE1
作者:Yu, Mengmeng;Zhang, Yao;Zhang, Li;Wang, Suyan;Liu, Yongzhen;Xu, Zhuangzhuang;Liu, Peng;Chen, Yuntong;Guo, Ru;Meng, Lingzhai;Zhang, Tao;Fan, Wenrui;Qi, Xiaole;Gao, Li;Zhang, Yanping;Cui, Hongyu;Gao, Yulong;Gao, Yulong;Gao, Yulong
关键词: