Development of gonad and brood pouch and expression profiles of cg α , fsh β and lh β transcripts and plasma 17 β-E2 and 11-KT during the first reproductive cycle of farmed big-belly seahorse ( Hippocampus abdominalis )

文献类型： 外文期刊

第一作者： Li, Yuru

作者： Li, Yuru;Zhao, Limiao;Jin, Weiqi;Luo, Renhui;Xu, Gang;Fang, Yan;Chen, Jun;Wang, Bin;Wang, Bin

作者机构：

关键词： Gonadotropins; Sex steroid; Growth of gonad and brood pouch; Seahorse

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2024 年 589 卷

页码：

收录情况： SCI

摘要： Limited data are available regarding the gonadal development of farmed seahorse along with the physiological role of gonadotropins and sex steroids during gonadal maturation. In the present study, we conducted an examination of the developmental stages of gonad and brood pouch during the initial reproductive cycle of the farmed big -belly seahorse ( Hippocampus abdominalis ), and our results showed that males sexually matured half of a month later than females, and the brood pouch emerged at 3.5 -month old and then reached the late -stage at 5.5 -month old. Meanwhile, the open reading frame (ORF) of gonadotropin subunits ( cg alpha , fsh beta and lh beta ) of the bigbelly seahorse were cloned and characterized, and tissue distribution profiles of their transcripts were analyzed, revealing their detectability across various tissues, with a notable presence observed in the brain tissues. Furthermore, expression profiles of cg alpha , fsh beta and lh beta transcripts and the plasma levels of 17 beta-estradiol (E2) and 11-ketotestosterone (KT) in farmed big -belly seahorse during the first reproductive cycle were also investigated. The findings indicated that cg alpha , fsh beta and lh beta transcripts exhibited low expressions at the initial development of ovary or testis, raised continuously and reached their peak levels at the period of spawning or spermation, and they kept gradually increasing accompany with brood pouch growth. It is noteworthy that variation of mRNA levels of fsh beta were slightly higher than those of lh beta . Besides, the results showed that plasma 11 -KT levels presented an increasing trend during testicular development, peaking in fish with III and IV -stage testis and earlystage brood pouch, and plasma 17 beta-E2 levels were higher in vitellogenic stage of fish with II -stage ovary and early III -stage ovary. Taken together, these data reveal that gonadotropins and sex steroids likely play a role in the regulation of gonad and brood pouch growth in seahorse. These observations not only contribute to clarifying function of reproductive hormones on growth of gonad and brood pouch of seahorse, but also providing an important theoretical foundation for the artificial breeding of seahorses.

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