Two Figla homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Li, Hailong

作者： Li, Hailong;Xu, Wenteng;Zhang, Ning;Shao, Changwei;Zhu, Ying;Dong, Zhongdian;Wang, Na;Jia, Xiaodong;Xu, Hao;Chen, Songlin;Li, Hailong;Xu, Wenteng;Zhang, Ning;Shao, Changwei;Zhu, Ying;Dong, Zhongdian;Wang, Na;Jia, Xiaodong;Xu, Hao;Chen, Songlin;Li, Hailong;Xu, Wenteng;Zhang, Ning;Shao, Changwei;Zhu, Ying;Dong, Zhongdian;Wang, Na;Jia, Xiaodong;Xu, Hao;Chen, Songlin

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： Figla is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of Figla is commonly found. However, besides the common form of this gene (named Figla_tv1), a new transcriptional form (named Figla_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of Figla_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while Figla_tv2 encoded a 125 amino acid protein. Figla_tv1 and Figla_tv2 expression in various tissues was detected by qRT-PCR. Figla_tv1 was expressed mainly in ovary, skin and liver, while Figla_tv2 was expressed in all examined tissues. In the gonads, Figla_tv1 was expressed in ovary, while Figla_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located Figla_tv1 only in oocytes and Figla_tv2 mainly in germ cells of pseudomale testis. After knocking down Figla_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly upregulated (P < 0.05). Our findings suggest that Figla_tv1 has a conserved function in folliculogenesis, as in other vertebrates, and that Figla_tv2 may have a role in the spermatogenesis of pseudomales by regulating the synthesis and metabolism of steroid hormones.

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