Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

文献类型： 外文期刊

第一作者： Rahman, N. M. A.

作者： Rahman, N. M. A.;Fu, H. T.;Jin, S.;Bai, H. K.;Liang, G. X.;Gong, Y. S.;Wu, Y.;Fu, H. T.;Sun, S. M.;Qiao, H.;Jin, S.;Zhang, W. Y.;Xiong, Y. W.;Rahman, N. M. A.

作者机构：

关键词： Macrobrachium nipponense;Nitric oxide synthase;Cloning;Expression pattern;RT-qPCR;Post-larvae

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 3 期

页码：

收录情况： SCI

摘要： Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of L-arginine to L-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponense is poorly understood. In this study, we cloned the full-length NOS complementary DNA from M. nipponense (MnNOS) and characterized its expression pattern in different tissues and at different developmental stages. Real-time quantitative polymerase chain reaction (RT-qPCR) showed the MnNOS gene to be expressed in all investigated tissues, with the highest levels observed in the androgenic gland (P < 0.05). Our results revealed that the MnNOS gene may play a key role in M. nipponense male sexual differentiation. Moreover, RT-qPCR revealed that MnNOS mRNA expression was significantly increased in post-larvae 10 days after metamorphosis (P < 0.05). The expression of this gene in various tissues indicates that it may perform versatile biological functions in M. nipponense.

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