Proteomic profiling of follicle fluids after superstimulation in one-month-old lambs

文献类型： 外文期刊

第一作者： Wu, Y.

作者： Wu, Y.;Lin, J.;Han, B.;Wang, L.;Chen, Y.;Liu, M.;Huang, J.

作者机构：

关键词： follicular fluid;lamb;proteome;quantitative proteomic;superstimulation

期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）

ISSN： 0936-6768

年卷期： 2018 年 53 卷 1 期

页码：

收录情况： SCI

摘要： Follicular fluid (FF) accumulates in the antrum of the ovarian follicle. In addition, FF provides the microenvironment for oocyte development, oocyte maturation and competence, which are acquired during follicular development. Superstimulatory treatment of 1-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles are unable to completely mature and ovulate. Furthermore, the oocytes exhibit lower competence compared with those of ewes. In this study, we utilized an isobaric tag for relative and absolute quantification (iTRAQ)-based proteomics analysis and compare protein composition between pre-pubertal and adult superstimulated follicle FF in sheep. In total, 243 differentially expressed proteins were identified, including 155 downregulated and 88 upregulated between lamb and ewe. Gene ontology (GO) and KEGG pathway analysis indicated that the differentially expressed proteins are involved in signal transduction, anatomical structure development, stress response, metabolic pathways, and the complement and coagulation cascades. Many of the proteins known to affect follicle development were observed in lower abundance in FF of lamb (e.g. ADAMTS9, CD14, CTNNB1, FST, GCLC, HSPG2, IGFBP2, IGFBP6, INHBA, PRL, PAPPA, POSTN, PRDX1, SERPINA1, SOD3, STC1, VEGFC, etc.). However, a higher abundance was observed for proteasome proteins. Inadequate amounts of these proteins in FF may be lead to the unique characteristics of follicular development in lamb. These differentially expressed proteins illuminate the age-dependent changes in protein expression in the follicle microenvironment.

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