文献类型： 外文期刊

第一作者： Liu, Ziyan

作者： Liu, Ziyan;Xu, Wenbo;Sui, Liyan;Zhao, Yinghua;Yin, Qing;Liu, Quan;Wang, Zedong;Liu, Ziyan;Liang, Xiaojie;Wei, Feng;Li, Liang;Zhang, Li;Li, Qianxue;Liu, Quan;Wang, Zedong;Yuan, Yongxu;Li, Dajun;Wei, Zhengkai;Liu, Quan;Cui, Yanyan;Hou, Zhijun

作者机构：

期刊名称：PLOS NEGLECTED TROPICAL DISEASES （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 1935-2735

年卷期： 2022 年 16 卷 12 期

页码：

收录情况： SCI

摘要： Background Ticks act as important vectors of infectious agents, and several emerging tick-borne viruses have recently been identified to be associated with human diseases in northeastern China. However, little is known about the tick virome in northeastern China. Methods Ticks collected from April 2020 to July 2021 were pooled for metagenomic analysis to investigate the virome diversity in northeastern China. Results In total, 22 RNA viruses were identified, including four each in the Nairoviridae and Phenuiviridae families, three each in the Flaviviridae, Rhabdoviridae, and Solemoviridae families, two in the Chuviridae family, and one each in the Partitiviridae, Tombusviridae families and an unclassified virus. Of these, eight viruses were of novel species, belonging to the Nairoviridae (Ji'an nairovirus and Yichun nairovirus), Phenuiviridae (Mudanjiang phlebovirus), Rhabdoviridae (Tahe rhabdovirus 1-3), Chuviridae (Yichun mivirus), and Tombusviridae (Yichun tombus-like virus) families, and five members were established human pathogens, including Alongshan virus, tick-borne encephalitis virus, Songling virus, Beiji nairovirus, and Nuomin virus. I. persulcatus ticks had significant higher number of viral species than H. japonica, H. concinna, and D. silvarum ticks. Significant differences in tick viromes were observed among Daxing'an, Xiaoxing'an and Changbai mountains. Conclusions These findings showed an extensive diversity of RNA viruses in ticks in northeastern China, revealing potential public health threats from the emerging tick-borne viruses. Further studies are needed to explain the natural circulation and pathogenicity of these viruses. Author summary Ticks are important vectors to transmit many infectious agents. In recent years, many novel tick-borne viruses associated with human illness, including Alongshan virus, Songling virus, and Beiji nairovirus, have been identified in northeastern China. It is necessary to conduct routine surveillance of tick-borne viruses in ticks, however, little is known about the tick virome in northeastern China. In this study, we used metatranscriptomics to investigate the virome diversity in four tick species collected from northeastern China. In total, we identified 22 RNA viruses belonging to eight virus families (exclude an unclassified virus). Of these, five members were established human pathogens, eight viruses were of novel species, and six viruses had close relationship with pathogenic tick-borne viruses with potential public health threats. Moreover, we found that the viromes were significantly affected by the tick species and geographical location in the study. These findings revealed an extensive diversity of RNA viruses in ticks in northeastern China, which will lay the foundation for the prevention and control of emerging tick-borne diseases.

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