Free Amino Acids Profile and Expression Analysis of Core Genes Involved in Branched-Chain Amino Acids Metabolism during Fruit Development of Longan (Dimocarpus longan Lour.) Cultivars with Different Aroma Types

文献类型： 外文期刊

第一作者： Hu, Wenshun

作者： Hu, Wenshun;Ali, Muhammad Moaaz;Chen, Faxing;Hu, Wenshun;Chen, Xiuping;Zheng, Shaoquan;Wang, Baiyu;Zhang, Jisen

作者机构：

关键词： fruit quality; L-alanine; L-leucine; BCAT; IPMS; IPMD

期刊名称：BIOLOGY-BASEL （ 影响因子：5.079； ）

ISSN：

年卷期： 2021 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Simple Summary In this study, three longan cultivars, including non-aroma types 'Shixia' (SX), 'Lidongben' (LDB), and strong aroma type 'Xiangcui' (XC), were selected to analyze free amino acids (FAAs) variations at six distinct growth stages. The genome-wide identification and expression analysis of genes related to the branched-chain amino acids (BCAA) synthesis pathway were carried out. Results showed that thirty-six FAAs were identified, which increased drastically with fruit development until ripening. During the period of rapid fruit expansion, the aroma of XC changed from light to strong, and the contents of L-alanine and L-leucine were significantly higher than those of SX and LDB. The content of Leu was negatively correlated with the expression of DilBCAT1, -6, and -9 in three varieties, but positively correlated with DilBCAT16, indicating that these four genes may be responsible for the different synthesis and degradation of Leu among cultivars. Amino acids are important component of fruit nutrition and quality. In this study, three longan cultivars, including non-aroma types 'Shixia' (SX), 'Lidongben' (LDB), and strong aroma type 'Xiangcui' (XC), were selected to analyze free amino acids (FAAs) variations at six distinct growth stages (S1-S6). The genome-wide identification and expression analysis of genes related to the branched-chain amino acids (BCAA) synthesis pathway were carried out. Results showed that 36 FAAs were identified, and the total FAAs content ranged from 2601.0 to 9073.5 mg/kg, which increased drastically with fruit development until ripening. L-glutamic acid (Glu), L-alanine (Ala), L-arginine (Arg), gamma-Aminobutyric acid (GABA), L-aspartic acid (Asp), L-leucine (Leu), hydroxyl-proline (Hypro), and L-serine (Ser) were the predominant FAAs (1619.9-7213.9 mg/kg) in pulp, accounting for 62.28-92.05% of the total amino acids. During the period of rapid fruit expansion (S2-S4), the aroma of XC changed from light to strong, and the contents of L-alanine (Ala) and L-leucine (Leu) were significantly higher than those of SX and LDB. Furthermore, a total of two 2-isopropyl malate synthase (IPMS), two 3-isopropyl malate dehydrogenase (IPMD), and 16 BCAA transferase (BCAT) genes were identified. The expression levels of DilBCAT1, -6, and -9 genes in XC were significantly higher than those in SX and LDB, while DilBCAT16 in XC was lower. The content of Leu was negatively correlated with the expression of DilBCAT1, -6, and -9 in three varieties, but positively correlated with DilBCAT16, indicating that these four genes may be responsible for the different synthesis and degradation of Leu among cultivars.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序