Keap1 Negatively Regulates Transcription of Three Counter-Defense Genes and Susceptibility to Plant Toxin Gossypol in Helicoverpa armigera

文献类型： 外文期刊

第一作者： Xie, Xingcheng

作者： Xie, Xingcheng;Wang, Qian;Liang, Gemei;Xie, Xingcheng;Deng, Zhongyuan;Xie, Xingcheng;Li, Xianchun;Li, Xianchun;Gu, Shaohua

作者机构：

关键词： counter-defense genes; gossypol; oxidative stress; Helicoverpa armigera; Keap1

期刊名称：INSECTS （ 影响因子：3.0； 五年影响因子：3.1 ）

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年卷期： 2024 年 15 卷 5 期

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收录情况： SCI

摘要： The Keap1-Nrf2-ARE signaling pathway has been suggested to induce the expressions of antioxidant and counter-defense genes, and aid cells in xenobiotic and oxidative responses. Nrf2 is the central transcription factor, and Keap1 is its specific repressor in this signaling pathway. To estimate the function of the Keap1 gene on plant toxin gossypol metabolism, we characterized the pathway in Helicoverpa armigera. The results demonstrated that the suppression of the Keap1 gene not only increased the expressions of three counter-defense genes CYP9A17, CYP4L11 and UGT41B3, but also reduced the larval mortality and promoted the larval development of those treated by the diet with gossypol. Our study showed that Keap1 negatively regulated the transcription of these three counter-defense genes and the knockdown of the Keap1 gene contributed to decreasing the susceptibility to gossypol in the cotton bollworm, which may be beneficial for further research on the regulation of insect counter-defense gene expression and insect-plant interactions. Expressions of a wide range of cytoprotective counter-defense genes are mainly regulated by the Keap1-Nrf2-ARE signaling pathway in response to oxidative stress from xenobiotics. Gossypol is the major antiherbivore secondary metabolite of cotton, but how the polyphagous pest Helicoverpa armigera copes with this phytochemical to utilize its favorite host plant cotton remains largely elusive. In this study, we first suppressed the Keap1 gene in newly hatched larvae of cotton bollworm by feeding them the siRNA diet for 4 days. All of the larvae were subsequently fed the artificial diet supplied with gossypol or the control diet for 5 days. We identified that the knockdown of the Keap1 gene significantly decreased larval mortality and significantly increased the percentages of larval survival, reaching the fourth instar, compared with ncsiRNA when exposed to a diet containing gossypol. Three counter-defense genes CYP9A17, CYP4L11 and UGT41B3, which were related to the induction or metabolism of gossypol according to the report before, were all significantly up-regulated after the knockdown of the Keap1 gene. The Antioxidant Response Elements (AREs) were also detected in the promoter regions of the three counter-defense genes above. These data indicate that the suppression of the Keap1 gene activates the Keap1-Nrf2-ARE signaling pathway, up-regulates the expressions of counter-defense genes involved in the resistance of oxidative stress and finally contributes to reducing the susceptibility of gossypol. Our results provide more knowledge about the transcriptional regulation mechanisms of counter-defense genes that enable the cotton bollworm to adapt to the diversity of host plants including cotton.

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