Acyclic Cucurbit[n]uril-Enabled Detection of Aflatoxin B1 via Host-Guest Chemistry and Bioluminescent Immunoassay

文献类型： 外文期刊

第一作者： Wu, Shaowen

作者： Wu, Shaowen;Niu, Jinlu;Xu, Jintao;Mo, Hualian;Yan, Shijuan;Feng, Ke;Yu, Shang-Bo;Li, Zhan-Ting;She, Xiaoman

作者机构：

关键词： aflatoxin B1; host-guest chemistry; acyclic cucurbit[n]urils; bioluminescent immunoassay; supramolecular recognition

期刊名称：TOXINS （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN：

年卷期： 2025 年 17 卷 3 期

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收录情况： SCI

摘要： Aflatoxin B1 (AFB1), a highly toxic secondary metabolite produced by Aspergillus species, represents a significant health hazard due to its widespread contamination of agricultural products. The urgent need for sensitive and sustainable detection methods has driven the development of diverse analytical approaches, most of which heavily rely on organic solvents, posing environmental challenges for routine food safety analysis. Here, we introduce a supramolecular platform leveraging acyclic cucurbit[n]uril (acCB) as a host molecule for environmentally sustainable AFB1 detection. Screening various acCB derivatives identified acCB6 as a superior host capable of forming a stable 1:1 complex with AFB1 in an aqueous solution, exhibiting a high binding affinity. Proton nuclear magnetic resonance (1H NMR) spectroscopy confirmed that AFB1 was deeply encapsulated within the host cavity, with isothermal titration calorimetry (ITC) experiments and molecular dynamics simulations further substantiating the stability of the interaction, driven by enthalpic and entropic contributions. This supramolecular host was incorporated into a scaffold-assembly-based bioluminescent enzyme immunoassay (SA-BLEIA), providing a green detection platform that rivals the performance of traditional organic solvent-based assays. Our findings highlight the potential of supramolecular chemistry as a foundation for eco-friendly mycotoxin detection and offer valuable insights into designing environmentally sustainable analytical methods.

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