RNA interference-based dsRNA application confers prolonged protection against rice blast and viral diseases, offering a scalable solution for enhanced crop disease management

文献类型： 外文期刊

第一作者： Chen, Pan

作者： Chen, Pan;Ding, Shaochen;Du, Ruonan;Hu, Xiaoxiao;Zhang, Han;Yu, Hanxi;Xu, Le;Niu, Dongdong;Liu, Hongxia;Zhao, Hongwei;Lan, Ying;Li, Chenyang;Lin, Feng;Du, Linlin;Zhou, Tong;Umida, Isashova;Ray, Rumiana;Liu, Tong;Liang, You

作者机构：

关键词： gene silence; MoPth11; plant protection; rice blast; rice stripe disease; RNAi; RSV; SIGS; small RNA

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.3； 五年影响因子：10.8 ）

ISSN： 1672-9072

年卷期： 2025 年 67 卷 6 期

页码：

收录情况： SCI

摘要： Rice production is severely impacted by pathogens such as Magnaporthe oryzae and the rice stripe virus (RSV). Ineffectiveness in controlling viruses and the excessive use of fungicides have proven traditional chemical pesticides increasingly inadequate. RNA interference (RNAi) represents a cutting-edge approach for combating crop diseases, especially in rice. This study addresses the critical gap in scalable, effective RNAi-based rice disease management by exploring the potential of spray-applied small RNA (sRNA) and double-stranded RNA (dsRNA) molecules. We utilized dsRNAs produced by in vitro transcription and bacterial expression systems and employed layered double hydroxides (LDH) to enhance RNA stability, absorption, and efficacy. Our research demonstrated that modified sRNAs could effectively penetrate M. oryzae cell membranes and inhibit conidial germination and appressorium formation, while LDH-conjugated dsRNAs provided prolonged and enhanced protection against both rice blast and rice stripe diseases. Most importantly, dsRNA treatments resulted in improved agronomic traits or increased crop yields by protecting against blast and stripe diseases. This study also validated the compatibility of these RNA molecules with industrial production methods, highlighting their potential as a scalable and eco-friendly option for managing crop diseases at the gene level. This work not only offers a new direction for rice disease control but also provides a foundation for the broader application of RNAi technology in agricultural pest management.

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