Preparation, Structural Characterization, and In Vitro Modulation of the Gut Microbiota Activity of a Novel α-Glucan in Hericium erinaceus

文献类型： 外文期刊

第一作者： Chen, Shuang

作者： Chen, Shuang;Liu, Liping;Feng, Jie;Zhang, Jingsong;Geng, Jie;Wang, Jinyan;Yang, Yan;Wu, Di;Liu, Yanfang;Chen, Shuang;Guo, Qingbin

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关键词： Hericium erinaceus; alpha-(1 -> 4)-glucan; structural characterization; in vitro digestion and fermentation; gut microbiota

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 14 期

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收录情况： SCI

摘要： Hericium erinaceus (H. erinaceus) is a kind of medicinal and edible fungus that contains polysaccharide as the most studied effective component at present. In this paper, a novel alpha-glucan was successfully isolated from H. erinaceus fruiting bodies by hot water extraction combined with the fractional ethanol precipitation method. To analyze the monosaccharide composition, methylation and nuclear magnetic resonance spectroscopy were performed, and the polysaccharide was identified having alpha-(1 -> 4)-glycosidic bonds with a single unit alpha-D-Glcp branch attached at the O-6 position (M w , 1.43 x 107 g/mol, branching ratio of 9:1). Furthermore, the aqueous solution analysis demonstrated that this alpha-glucan adopts a rigid spherical conformation. The polysaccharide exhibited resistance to simulated gastrointestinal digestion but was effectively degraded by gut microbiota (GM), leading to significant changes in total polysaccharide content, reducing sugar, and pH value. Notably, the alpha-glucan was beneficial to increase the contents of acetic and propionic acids in metabolites, stimulated the growth of beneficial bacteria (Lactobacillus and Bifidobacterium), and inhibited the proliferation of harmful bacteria (Fusobacterium). Overall, the study deepened the regulatory effects of alpha-glucan with different ratios of main and branch chains from H. erinaceus fruiting bodies on GM, which provides theoretical support for developing of GM-targeted foods.

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