Formation of Proto-Kranz in C3 Rice Induced by Spike-Stalk Injection Method

文献类型： 外文期刊

第一作者： Jiang, Dexing

作者： Jiang, Dexing;Wang, Feng;Zhang, Haizi;Gao, Wenwen;Tong, Xi;Chen, Guoxiang;Wang, Feng;Lv, Chuangen

作者机构：

关键词： C4 rice; proto-Kranz; photosynthetic efficiency; crop improvement; spike-stalk injection

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.924； 五年影响因子：6.132 ）

ISSN：

年卷期： 2021 年 22 卷 9 期

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收录情况： SCI

摘要： Introduction of C4 photosynthetic traits into C3 crops is an important strategy for improving photosynthetic capacity and productivity. Here, we report the research results of a variant line of sorghum-rice (SR) plant with big panicle and high spikelet density by introducing sorghum genome DNA into rice by spike-stalk injection. The whole-genome resequencing showed that a few sorghum genes could be integrated into the rice genome. Gene expression was confirmed for two C4 photosynthetic enzymes containing pyruvate, orthophosphate dikinase and phosphoenolpyruvate carboxykinase. Exogenous sorghum DNA integration induced a series of key traits associated with the C4 pathway called "proto-Kranz" anatomy, including leaf thickness, bundle sheath number and size, and chloroplast size in bundle sheath cells. Significantly, transgenic plants exhibited enhanced photosynthetic capacity resulting from both photosynthetic CO2-concentrating effect and improved energy balance, which led to an increase in carbohydrate levels and productivity. Furthermore, such rice plant exhibited delayed leaf senescence. In summary, this study provides a proof for the feasibility of inducing the transition from C3 leaf anatomy to proto-Kranz by spike-stalk injection to achieve efficient photosynthesis and increase productivity.

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