Nocardia rosealba sp. nov., a novel ligninase-producing Actinobacterium isolated from soil

文献类型： 外文期刊

第一作者： Huang, Zhenzhen

作者： Huang, Zhenzhen;He, Chuan;Wang, Zishan;Luo, Xianxian;Sun, Xiujun;Zhao, Junwei;Gao, Xingyu;Xiang, Wensheng;Song, Jia;Wang, Xiangjing;Xiang, Wensheng

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关键词： Nocardia rosealba sp. nov.; polyphasic taxonomy; 16S rRNA gene sequence; whole-genome analysis

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.689； 五年影响因子：2.925 ）

ISSN： 1466-5026

年卷期： 2022 年 72 卷 6 期

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收录情况： SCI

摘要： A novel ligninase-producing actinomycete, designated strain NEAU-G4(T), was isolated from a soil sample and subjected to a polyphasic taxonomic study to establish its status. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Nocardia, with the highest sequence similarity to Nocardia ignorata DSM 44496(T) (99.2%). The whole-cell sugars contained galactose and arabinose. The amino acid of the cell wall was determined to be meso-diaminopimelic acid. The major fatty acids (>10%) were C-16:0, C-18:1 omega 9c, C-18:0 and C-16:1 omega 7c. The predominant menaquinone was identified as MK-8(H 6, omega-cycl). The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Strain NEAU-G4(T) had a draft genome size of 6405167 bp, annotated with 5815 protein-coding genes. The DNA G+C content was 67.6 mol%. Phylogenetic analysis using the 16S rRNA gene and whole-genome sequences showed that strain NEAU-G4(T) formed a stable phyletic line with N. ignorata DSM 44496(T). The digital DNA-DNA hybridization and average nucleotide identity values between them were 63.7% (60.8-66.5%) and 95.5%, respectively. Moreover, genomic analysis indicated that strain NEAU-G4(T) had the potential to degrade lignin and produce bioactive compounds. On the basis of genotypic analysis, physiological data, as well as phenotypic and chemotaxonomic characterizations, it is concluded that the organism be classified as representing a novel species of the genus Nocardia, for which the name Nocardia rosealba sp. nov. is proposed. The type strain is NEAU-G4(T) (=CCTCC AA 2020038(T)=DSM 111936(T)).

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