文献类型： 外文期刊

第一作者： Zhang, Xiaoming

作者： Zhang, Xiaoming;Song, Tianzeng;Zhaxi, Yangzong;Chen, Xiaoying;Zhang, Xiaoming;Wu, Jing;Mustafa, Shehr Bano;Zhao, Wangsheng;Song, Tianzeng;Liu, Guiqiong;Jiang, Xunping;Shahzad, Khuram

作者机构：

关键词： male Xizang sheep; GnRH immunocastration; rumen; microorganisms

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 20 期

页码：

收录情况： SCI

摘要： Simple Summary Castration is a routine procedure in sheep farming, and immunocastration is beginning to be used in animal production as an alternative to surgical castration. Currently, there are few studies on the intra-rumen environment after immunocastration in sheep. Therefore, in this study, male Xizang sheep were selected for surgical castration and GnRH immunocastration treatment, the intact control group and immunocastrated group were selected in combination with body weight data, and microbiology and metabolomics techniques were used to analyze the effects of GnRH immunocastration on the rumen internal environment of male Xizang sheep. The results showed that GnRH immunocastration had a more obvious effect on body weight gain while achieving the effect of de-population; immunocastration increased the ratio of rumen thick-walled bacterial phylum to anamorphic bacterial phylum, which improved the digestive utilization of forage feed in male Xizang sheep. Secondly, GnRH immunocastration can maintain the balance of the rumen internal environment and the health of rumen epithelial cells by improving the levels of certain metabolites in the rumen.Abstract Castration is a prevalent and indispensable practice in sheep husbandry, aiding in enhancing meat quality, mitigating aggressive behavior, and managing unwanted reproduction. Nevertheless, the conventional surgical castration procedure poses several challenges, including heightened stress and pain, detrimental impacts on animal welfare, and diminished economic efficacy in farming operations. Consequently, immunocastration methods, serving as substitutes for surgical castration, are progressively finding application in livestock. The rumen, an essential and distinctive digestive and absorptive organ in ruminants, has been associated with enhanced meat quality and productive performance following castration in previous research studies, albeit fewer investigations have explored the potential impacts of GnRH immunization on the rumen's internal milieu in sheep post-de-escalation. Hence, the present study delved into evaluating the impact of GnRH immunocastration on the rumen microbiome and metabolomics in male Xizang sheep. This was achieved through the establishment of a GnRH immunocastration animal model and the collection of rumen fluid for microbiological and comprehensive metabolomics investigations. The outcomes of this investigation unveiled that the impact of GnRH immunocastration on body weight gain was more pronounced during the achievement of the castration objective. In addition, the Firmicutes-to-Bacteroidota ratio in the immune male (IM) group exceeded that of the control group (EM), suggesting that GnRH immunodeficiency may enhance the digestion and absorption of feed in male Xizang sheep. At the taxonomic level, the elevated presence of Prevotella and Quinella bacteria in the IM group compared to the EM group indicated that castration influenced a segment of the rumen microbiota in male Xizang sheep, thereby bolstering the digestive and metabolic efficacy of the rumen concerning nutrient utilization, particularly in the breakdown and absorption of proteins, carbohydrates, and lipids, ultimately expediting the fattening process and weight gain in male Xizang sheep following castration. Moreover, analysis of ruminal fluid metabolomics revealed that GnRH immunization had notable impacts on certain metabolites in the ruminal fluid of male Xizang sheep, with metabolites like 5-hydroxyindole acetic acid and 3-hydroxyindole acetic acid showing significant downregulation in the IM group compared to the EM group, while niacin and tyramine exhibited significant upregulation. These findings indicate a profound influence of GnRH immunization on the maintenance of ruminal equilibrium and ruminal health (including the health of ruminal epithelial cells). This study validates that GnRH immunocastration not only achieves the objectives of castration but also enhances ruminal health in male Xizang sheep, thus laying a foundational theoretical basis for the application and dissemination of GnRH immunocastration technology.

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