Ascorbic acid-mediated in situ growth of gold nanostars for photothermal immunoassay of ochratoxin A

文献类型： 外文期刊

第一作者： Wang, Yiwen

作者： Wang, Yiwen;Xie, Longyingzi;Ma, Lanrui;Wu, Qi;Liu, Yanlin;Zhao, Qiyang;Zhang, Yaohai;Jiao, Bining;He, Yue;Wang, Yiwen;Xie, Longyingzi;Ma, Lanrui;Wu, Qi;Liu, Yanlin;Zhao, Qiyang;Zhang, Yaohai;Jiao, Bining;He, Yue;Wang, Yiwen;Ma, Lanrui;Wu, Qi;Li, Zhixia;He, Yue;Li, Zhixia

作者机构：

关键词： Photothermal immunoassay; Gold nanostars; Tyramine signal amplification; Portable detection; Ochratoxin A

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 419 卷

页码：

收录情况： SCI

摘要： Currently, the development of efficient mycotoxins detection methods, particularly using portable devices as readout devices, remains a great challenge. Herein, a photothermal enzyme-linked immunosorbent assay (ELISA) based on gold nanostars (AuNSs) for the detection of ochratoxin A (OTA) using a "thermometer" was proposed for the first time. AuNSs with photothermal conversion capacity were parepared using an ascorbic acid (AA) -mediated in situ growth methd. Quantification was based on the alkaline phosphatase catalyzing the dephos-phorylation of ascorbic acid 2-phosphoate to AA, thereby converting OTA concentration to the amount of in situ synthesized AuNSs, thus achieving straightforward readout by temperature. Benefiting from the classical tyra-mine signal amplification strategy, a detection limit of 0.39 ng mL-1 was obtained. The recoveries of grape juice and maize samples spiked with 10 ng mL-1 and 30 ng mL-1 OTA ranged from 86.53% to 116.9%. Our method has great potential in on-site OTA detection for food safety.

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