Controllable self-cleaning FET self-assembled RNA-cleaving DNAzyme based DNA nanotree for culture-free Staphylococcus aureus detection

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Chen, Ruipeng;He, Yue;Zhu, Xiaoyan;Yu, Zhixue;Pan, Dongxia;Yang, Liang;Tang, Xiangfang;Xiong, Benhai;Feng, Zemeng;He, Yue

作者机构：

关键词： Self-cleaning field effect transistor; DNA origami; Electrochemical biosensor; Carbon nanotube; Superhydrophobic-oleophobic coating

期刊名称：JOURNAL OF NANOBIOTECHNOLOGY （ 影响因子：10.6； 五年影响因子：11.4 ）

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年卷期： 2024 年 22 卷 1 期

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收录情况： SCI

摘要： Staphylococcus aureus (SA) poses a serious risk to human and animal health, necessitating a low-cost and high-performance analytical platform for point-of-care diagnostics. Cellulose paper-based field-effect transistors (FETs) with RNA-cleaving DNAzymes (RCDs) can fulfill the low-cost requirements, however, its high hydrophilicity and lipophilicity hinder biochemical modification and result in low sensitivity, poor mechanical stability and poor fouling performance. Herein, we proposed a controllable self-cleaning FET to simplify biochemical modification and improve mechanical stability and antifouling performance. Then, we constructed an RCD-based DNA nanotree to significantly enhance the sensitivity for SA detection. For controllable self-cleaning FET, 1 H,1 H,2 H,2 H-perfluorodecyltrimethoxysilane based-polymeric nanoparticles were synthesized to decorate cellulose paper and whole carbon nanofilm wires. O-2 plasma was applied to regulate to reduce fluorocarbon chain density, and then control the hydrophobic-oleophobic property in sensitive areas. Because negatively charged DNA affected the sensitivity of semiconducting FETs, three Y-shaped branches with low-cost were designed and applied to synthesize an RCD-based DNA-Nanotree based on similar DNA-origami technology, which further improved the sensitivity. The trunk of DNA-Nanotree was composed of RCD, and the canopy was self-assembled using multiple Y-shaped branches. The controllable self-cleaning FET biosensor was applied for SA detection without cultivation, which had a wide linear range from 1 to 10(5) CFU/mL and could detect a low value of 1 CFU/mL.

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