Effects of Recombinant Leptin Proteins on the Expression of Key Genes in the HPG Axis and Liver of Tongue Sole In Vitro

文献类型： 外文期刊

第一作者： Cai, Xin

作者： Cai, Xin;Zhang, Yaxing;Wang, Bin;Cui, Aijun;Jiang, Yan;Meng, Zhaojun;Li, Yuting;Xu, Yongjiang

作者机构：

关键词： leptin; HPG axis; tongue sole; in vitro; fish growth; reproduction; liver

期刊名称：FISHES （ 影响因子：2.3； 五年影响因子：2.4 ）

ISSN：

年卷期： 2023 年 8 卷 12 期

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收录情况： SCI

摘要： Leptin (Lep) plays a crucial role in controlling food intake and maintaining energy balance in mammals. While several studies have shown the presence of multiple leptin genes in teleosts, limited information is currently available on how sex steroid hormones regulate the expression of these genes in fish. In this study, we used two previously expressed and purified leptin proteins to incubate four tissues in vitro (hypothalamic-pituitary-gonadal axis and liver) and used the RT-qPCR method to detect the expression of genes related to growth and reproduction in tongue sole (Cynoglossus semilaevis). The results showed that both recombinant LepA and LepB proteins of tongue sole almost entirely suppressed the expression of genes related to growth and reproduction in the hypothalamic-pituitary-gonadal axis and liver, while LepB may have had a positive role on steroid synthesis in gonads. Further, a high concentration of LepA facilitated the expression of IGF-Iota in liver. At the same time, we utilized human leptin to incubate four tissues in vitro; although most of them had trends similar to those stimulated with tongue sole leptins, there were still some differences, indicating differences among leptin homologs between humans and fishes. To our knowledge, this is the first study to explore the function of tongue sole LepA and LepB within the hypothalamic-pituitary-gonadal axis and liver in vitro. Our results provide a valuable resource and foundation for future studies.

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