Multi-omic analysis to develop key genes involved in carotenoid synthesis and establish indicative molecular markers for oil palm fruit colour

文献类型： 外文期刊

第一作者： Zhang, Ruimin

作者： Zhang, Ruimin;Liu, Xiaoyu;Cheng, Yang;Martin, Jerome Jeyakumar John;Li, Xinyu;Zhou, Lixia;Fu, Dengqiang;Li, Qihong;Li, Rui;Cao, Hongxing;Zhang, Ruimin;Liu, Xiaoyu;Cheng, Yang;Martin, Jerome Jeyakumar John;Li, Xinyu;Zhou, Lixia;Fu, Dengqiang;Li, Qihong;Li, Rui;Cao, Hongxing;Cheng, Yang

作者机构：

关键词： oil palm; nigrescens; virescens; Metabolome; Transcriptome; Carotenoids; Kompetitive Allele-Specific PCR molecular; marker

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 225 卷

页码：

收录情况： SCI

摘要： Oil palm (Elaeis guineensis Jacq.) is an important tropical woody oil-bearing economic crop. The outer skin of the fruit is rich in carotenoids, which are not only involved in the formation of oil palm skin color, but also confer antioxidative, anti-aging, and immune-regulating properties to palm oil. In this study, outer skin of virescens oil palm fruits (CS) from OxG Amazon and nigrescens oil palm fruits (CT) from DelixGhana as experimental materials after pollination at 95 days (CS1, CT1), 125 days (CS2, CT2), and 185 days (CS3, CT3). The changes in total carotenoid content and differences in metabolome and transcriptome at different developmental stages were analyzed, and SNPs mutation sites of related genes were selected to develop KASP molecular markers that can distinguish virescens and nigrescens oil palm. The results showed that the outer skin content of two species of oil palms varied significantly in terms of the total carotinoid content at various developmental stages. The virescens oil palm variety was found to contain a diverse range of carotenoids, with a total of 48 types identified. Similarly, the nigrescens oil palm variety was found to contain 52 types of carotenoids. The six types with the highest carotinoid content were: alpha-Carotenoids (C1), lycopenes (C2) gamma- Carotenoids (C4) beta- Carotenoids (C5), lycopene (C6), lutein (C38); Preliminary identification of 9 candidate genes in the carotenoid synthesis pathway of oil palm peel. In CS and CT, the PSY2 gene, PDS, ZDS, and LCYB were upregulated during fruit growth and development, whereas DWARF27, PSY2 gene, and LCYE were downregulated. CRTZ is downregulated in CS and upregulated in CT during fruit development; Three pairs of markers (K1, K2, and K3) were successfully developed to match the genotypes and corresponding phenotypes of 30 virescens oil palms and 30 nigrescens oil palms in the field with 100 % accuracy. These three pairs of markers are closely linked to the PSY2 gene and can effectively differentiate between virescens and nigrescens oil palms This study can provide the basis for elucidating the carotenoid biosynthesis process in oil palm outer skin in the future, as well as technical means for fruit type identification, variety purity testing, and screening of high content oil palm varieties with carotenoids during seedling phase of oil palm.

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