Precursor Supply Increases the Accumulation of 4-Hydroxy-6-(4-hydroxyphenyl)-alpha-pyrone after NRPS-PKS Gene Expression
文献类型： 外文期刊
第一作者： Li, Wen
作者： Li, Wen;Fan, Jie;Liao, Ge;Li, Shu-Ming;Fan, Jie;Yin, Wen-Bing;Liao, Ge
作者机构：
期刊名称：JOURNAL OF NATURAL PRODUCTS （ 影响因子：4.05； 五年影响因子：4.603 ）
ISSN： 0163-3864
年卷期： 2021 年 84 卷 8 期
页码：
收录情况： SCI
摘要： Expression of a nonribosomal peptide synthetase-nonreducing polyketide synthase hybrid gene pcr10109 from Penicillium crustosum PRB-2 in Aspergillus nidulans led to the accumulation of 4-hydroxy-6-(4-hydroxyphenyl)-alpha-pyrone (1). Adding para-hydroxybenzoic acid into the medium in which the overexpressing mutant is growing increased the product yield up to 5-fold. This strategy could be helpful for heterologous gene expression experiments requiring special substrates for product formation.
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