Simultaneous determination of somatic cell count and total plate count in raw milk based on ATP bioluminescence assay
文献类型： 外文期刊
第一作者： Yang, Longrui
作者： Yang, Longrui;Sun, Xiaoyun;Chen, Jiaci;Zhang, Juan;Li, Xiaoyu;Huang, Fengchun;Chen, Ailiang;Qu, Song;Wu, Kai
作者机构：
关键词： ATP; Bioluminescence assay; Raw milk; Somatic cell count; Total plate count
期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.0； 五年影响因子：5.7 ）
ISSN： 0003-2670
年卷期： 2024 年 1331 卷
页码：
收录情况： SCI
摘要： The somatic cell count (SCC) and total plate count (TPC) are essential quality indicators for raw milk. Traditional detection methods require separate measurements and rely on complex, large-scale instruments or cultivation techniques, which are both time-consuming and laborious. To address these challenges, this study developed a novel method for the simultaneous detection of SCC and TPC in the same raw milk sample using the ATP bioluminescence assay. This method utilizes oxy-ethylated iso-nonyl phenol (Neonol-10) and cetyltrimethylammonium bromide (CTAB) to selectively lyse somatic cells and microorganisms, respectively. This technique is straightforward to operate and can be completed within 2.5 h, with detection ranges of 1 x 104 to 3 x 106 cells/mL for SCC and 1 x 105 to 5 x 107 CFU/mL for TPC. Importantly, this technique meets the requirements of detection standards in China, European Union, Canada, United States, etc. For SCC or TPC in raw
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