Initiation of two ovarian cell lines from Fugu rubripes (Temminck et. Schlegel)

文献类型： 外文期刊

第一作者： Zheng Debin

作者： Zheng Debin;Pan Luqing;Zhang Bo;Song Wenping;Ma Chao;Xiao Guangxia

作者机构：

关键词： Fugu rubripes;ovary cell line;GFP;gene transfer;cytopathic effects;virus susceptibility

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2015 年 34 卷 10 期

页码：

收录情况： SCI

摘要： The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes (Temminck et. Schlegel) as a marine fish model of aquaculture importance, we established cell lines called TSOC1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like cells. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.

分类号：

  • 相关文献

[1]Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert. Wang, N.,Wang, X-L,Sha, Z-X,Tian, Y-S,Chen, S-L,Chen, S-L. 2011

[2]Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis. Zhang, Bo,Wang, Xianli,Sha, Zhenxia,Yang, Changgeng,Liu, Shanshan,Wang, Na,Chen, Song-Lin,Zhang, Bo,Liu, Shanshan.

[3]Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Wang, N.,Wang, X. L.,Sha, Z. X.,Tian, Y. S.,Chen, S. L..

[4]Sperm nuclear transfer and transgenic production in the fish medaka. Liu, Tongming,Hong, Yunhan,Liu, Ling,Wei, Qiwei,Hong, Yunhan.

[5]Retention of the developmental pluripotency in medaka embryonic stem cells after gene transfer and long-term drug selection for gene targeting in fish. Hong, YH,Chen, SL,Gui, JF,Schartl, M. 2004

[6]Transfer of stripe rust resistance from Aegilops variabilis to bread wheat. Zhang, Huaigang,Liu, Dengcai,Xiang, Zhiguo,Zhang, Lianquan,Zheng, Youliang,Chen, Guoyue,Wan, Chunjie,Liu, Dengcai,Zhang, Lianquan,Zheng, Youliang,Chen, Guoyue,Yang, Wuyun. 2011

[7]Gene duplication, transfer, and evolution in the chloroplast genome. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Gao, Feng,Zhu, Bo,Fu, Xiao-Yan,Xue, Yong,Jin, Xiao-Feng,Tian, Yong-Sheng,Zhao, Wei,Yao, Quan-Hong.

[8]Genetic Diversity of Nitrogen-Fixing and Plant Growth Promoting Pseudomonas Species Isolated from Sugarcane Rhizosphere. Singh, Rajesh K.,Singh, Pratiksha,Song, Qi-Qi,Xing, Yong-Xiu,Yang, Li-Tao,Li, Yang-Rui,Li, Yang-Rui. 2017

[9]Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Sha Zhenxia,Ren Guocheng,Wang Xianli,Wang Na,Chen Songlin,Ren Guocheng,Wang Xianli. 2010

[10]Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara. Liu, X. F.,Wu, Y. H.,Wang, N.,Li, Y. Z.,Zhang, N. W.,Chen, S. L.,Liu, X. F.,Chen, S. L.,Liu, X. F.,Wei, S. N.,Li, P. F.,Qin, Q. W.. 2018

[11]Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Wang, X. L.,Wang, N.,Sha, Z. X.,Chen, S. L.,Wang, X. L.. 2010

[12]Determination of stage interconversion in vitro and in vivo by construction of transgenic Toxoplasma gondii that stably express stage-specific fluorescent proteins. Zhang, Houshuang,Zhang, Yanlei,Cao, Jie,Zhou, Yongzhi,Wang, Na,Zhou, Jinlin. 2013

[13]Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita. Yao, Yu-Rong,Tian, Xue-Liang,Shen, Bao-Ming,Mao, Zhen-Chuan,Chen, Guo-hua,Xie, Bing-Yan,Tian, Xue-Liang,Shen, Bao-Ming.

[14]Microscopic analysis of the compatible and incompatible interactions between Fusarium oxysporum f. sp conglutinans and cabbage. Li, Erfeng,Wang, Gang,Yang, Yuhong,Xiao, Jiling,Mao, Zhenchuan,Xie, Bingyan.

[15]GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A.. 2016

[16]A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae. Ishag, H. Z. A.,Liu, M. J.,Yang, R. S.,Xiong, Q. Y.,Feng, Z. X.,Shao, G. Q.,Ishag, H. Z. A.. 2016

[17]A Viral Suppressor P1/HC-Pro Increases the GFP Gene Expression in Agrobacterium-mediated Transient Assay. Ma, Pengda,Liu, Jinying,He, Hongxia,Yang, Meiying,Li, Meina,Zhu, Xiaojuan,Wang, Xingzhi,Ma, Pengda,Yang, Meiying. 2009

[18]Expression of a novel bi-directional Brassica napus promoter in soybean. Chennareddy, Siva,Cicak, Toby,Clark, Lauren,Russell, Sean,Skokut, Michiyo,Beringer, Jeffrey,Jia, Yi,Gupta, Manju,Yang, Xiaozeng.

[19]Fluorescent transgenic silkworm. Zhang, F,Zhao, Y,Chen, X,Xu, AY,Huang, JT,Lu, CD. 1999

[20]Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination. Zhao, Y,Chen, X,Peng, WP,Dong, L,Huang, JT,Lu, CD. 2001

作者其他论文 更多>>

微信小程序