Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

文献类型： 外文期刊

第一作者： Wang, Gang

作者： Wang, Gang;He, Dan;Hu, Junqiang;Shi, Jianrong;Xu, Jianhong;Wang, Gang;Shi, Jianrong;Xu, Jianhong;Zhao, Fengchun;Lee, Yin-Won

作者机构：

关键词： Ustiloxin A; Rice false smut; Preparative purification; Macroporous resin; High-speed counter-current chromatography; Phytotoxicity assay

期刊名称：FOOD PRODUCTION PROCESSING AND NUTRITION （ 影响因子：4.7； 五年影响因子：5.0 ）

ISSN：

年卷期： 2020 年 2 卷 1 期

页码：

收录情况： SCI

摘要： Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay.

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