Two molecular markers based on mitochondrial genomes for varieties identification of the northern snakehead (Channa argus) and blotched snakehead (Channa maculata) and their reciprocal hybrids

文献类型： 外文期刊

第一作者： Zhang Xincheng

作者： Zhang Xincheng;Chen Kunci;Zhu Xinping;Zhao Jian;Luo Qing;Hong Xiaoyou;Li Wei;Xiao Fengfang;Zhang Xincheng;Chen Kunci;Zhu Xinping;Zhao Jian;Li Wei;Xiao Fengfang

作者机构：

关键词： Channa argus;Channa maculata;hybrids;mtDNA;molecular marker;varieties identification

期刊名称：MITOCHONDRIAL DNA （ 影响因子：0.925； 五年影响因子：0.994 ）

ISSN： 1940-1736

年卷期： 2015 年 26 卷 4 期

页码：

收录情况： SCI

摘要： The northern snakehead (Channa argus) and blotched snakehead (Channa maculata) and their reciprocal hybrids have played important roles in the Chinese freshwater aquaculture industry, with an annual production in China exceeding 400 thousand tons. While these are popular aquaculture breeds in China, it is not easy to identify northern snakehead, blotched snakehead, and their hybrids. Thus, a method should be developed to identify these varieties. To distinguish between the reciprocal hybrids (C. argus female x C. maculata male and C. maculata female x C. argus male), the mitochondrial genome sequences of northern snakehead and blotched snakehead and their reciprocal hybrids were compared. Following the alignment and analysis of mtDNA sequences of northern snakehead, blotched snakehead and their hybrids, two pairs of specific primers were designed based on identified differences ranging from 12S rRNA to 16S rRNA gene. The BY1 primers amplified the same bands in the blotched snakehead and the hybrid (C. maculata female x C. argus male), while producing no products in northern snakehead and the hybrid (C. argus female x C. maculata male). Amplification with WY1 yielded the opposite results. Then, 30 individuals per fish were randomized to verify the primers, and the results showed that the primers were specific for breeds, as intended. The specific primers can not only simply distinguish between two kinds of hybrids, but also rapidly identify the two parents. This study provides a method of molecular marker identification to identify reciprocal hybrids.

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