Genome-wide analysis of G-quadruplex in Spodoptera frugiperda

文献类型： 外文期刊

第一作者： Deng, Zhongyuan

作者： Deng, Zhongyuan;Ren, Yudong;Wang, Lixiang;Guo, Lina;Xie, Xingcheng;Li, Xianchun;Li, Xianchun

作者机构： Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450001, Henan, Peoples R China;Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA;Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA

关键词： G-quadruplex; Spodoptera frugiperda; DNA secondary structure; Pest control; NMM

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 2022影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 226 卷

页码：

收录情况： SCI

摘要： Spodoptera frugiperda (Lepidoptera: Noctuidae) is a globally distributed lepidopteran crop pest that has developed resistance to most insecticides. The G-quadruplex (G4) is a secondary structure in the genome enriched in the promoters for regulating gene expression. However, little is known about G4 in S. frugiperda, especially whether G4 is involved in insecticide resistance and pest control. In this study, 387,875 G4 motifs in the whole genome of S. frugiperda were identified by bioinformatics prediction. We found that 66.90 % of theseG4 structures were located in genic regions and highly enriched in the upstream regions of start codons. Functional and pathway analyses showed that the genes with G4 enriched in promoter regions participate in several metabolic processes. Further analyses showed that G4 structures occurred more frequently in the promoters of P450 and CarE gene families. It was also investigated that G4 ligand N-methyl mesoporphyrin IX (NMM) decreased P450 protein activity in larval midgut tissue. Cytotoxicity and bioassay results revealed that NMM and pesticides had syn-ergistic effects on toxicity. In conclusion, our findings suggest that G4 motif could be a new potential target for pest control.

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