CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus

文献类型： 外文期刊

第一作者： Zhou, Hongyu

作者： Zhou, Hongyu;Tang, Feng;Zhou, Hongyu;Bu, Shengjun;Xu, Yao;Xue, Lulu;Li, Zhongyi;Hao, Zhuo;Wan, Jiayu

作者机构：

关键词： Influenza H1N1 virus detection; CRISPR/Cas13a; Hybridization chain reaction; DNAzyme

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.478； 五年影响因子：4.29 ）

ISSN： 1618-2642

年卷期： 2022 年 414 卷 29-30 期

页码：

收录情况： SCI

摘要： This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-) of the probe, further initiating HCR to copiously generate G-rich DNA. Abundant G-quadruplex/hemin was formed in the presence of hemin, thus catalyzing a colorimetric reaction. The colorimetric biosensor exhibited a linear relationship from 10 pM to 100 nM. The detection limit was 0.152 pM. The biosensor specificity was excellent. This new and sensitive detection method for influenza virus is a promising rapid influenza diagnostic test.

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