Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products
文献类型： 外文期刊
第一作者： Qu, Meng
作者： Qu, Meng;Jiang, Yanhua;Guo, Yingying;Zhu, Wenjia;Li, Na;Li, Fengling;Yao, Lin;Tan, Zhijun;Wang, Lianzhu;Qu, Meng;Jiang, Yanhua;Guo, Yingying;Zhu, Wenjia;Liu, Shufang;Li, Na;Li, Fengling;Yao, Lin;Tan, Zhijun;Wang, Lianzhu;Liu, Shufang;Yao, Lin
作者机构：
关键词： Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene
期刊名称：JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY （ 影响因子：1.6； 五年影响因子：1.9 ）
ISSN： 1049-8850
年卷期： 2023 年 32 卷 10 期
页码：
收录情况： SCI
摘要： By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng mu L-1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg(-1). The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.
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