Taraxacum mongolicum Hand.-Mazz. derived extracellular vesicles alleviate mastitis via NLRP3 inflammasome and NF-κB/MAPK pathways

文献类型： 外文期刊

第一作者： Sun, Yuan

作者： Sun, Yuan;Liu, Ying;Li, Jinxian;Du, Yiyang;Chen, Danyang;Yang, Min;Peng, Yinghua;Huang, Shan

作者机构：

期刊名称：JOURNAL OF MATERIALS CHEMISTRY B （ 影响因子：5.7； 五年影响因子：6.1 ）

ISSN： 2050-750X

年卷期： 2025 年 13 卷 34 期

页码：

收录情况： SCI

摘要： Mastitis, a prevalent inflammatory disease affecting both humans and animals, imposes significant health burdens globally. Taraxacum mongolicum Hand.-Mazz. has been traditionally used to treat mammary gland disorders, however, the clinical translation of its crude extracts remains challenging due to the poor bioavailability. Emerging as innovative nanotherapeutic agents, plant-derived extracellular vesicles (PEVs) exhibit enhanced bioavailability, low immunogenicity, and targeted delivery capabilities, making them promising candidates for precision medicine applications. Herein, extracellular vesicles derived from Taraxacum mongolicum Hand.-Mazz. (TH-EVs) were successfully isolated employing ultracentrifugation and sucrose gradient centrifugation. Subsequently, these physicochemical properties, including particle size distribution and composition analysis, were comprehensively characterized. The anti-inflammatory efficacy and mechanism of TH-EVs were explored in both lipopolysaccharide (LPS)-stimulated murine mammary epithelial cells (HC11) and a murine mastitis model. In vitro, TH-EVs reduced TNF-alpha, IL-6, IL-1 beta and cellular oxidative stress. In vivo, TH-EVs alleviated histopathological damage, decreased myeloperoxidase activity, inhibited T lymphocyte activation, and reduced oxidative stress in mammary tissues. Mechanistically, TH-EVs inhibited the NLRP3 inflammasome, NF-kappa B and MAPK pathways. This study demonstrates that TH-EVs are a potent natural nanotherapeutic agent for mastitis, with potential for translational applications.

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