Metabolism-Based Herbicide Resistance to Mesosulfuron-methyl and Identification of Candidate Genes in Bromus japonicus

文献类型： 外文期刊

第一作者： Li, Qi

作者： Li, Qi;Yu, Jinping;Zhang, Wei;Liu, Yixue;Wang, Hengzhi;Guo, Wenlei

作者机构： Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin 300381, Peoples R China;Shandong Agr Univ, Coll Plant Protect, Key Lab Pesticide Toxicol & Applicat Tech, Tai An 271018, Peoples R China;Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

关键词： abiotic stress; Bromus japonicus; mesosulfuron-methyl; metabolic resistance; transcriptomics; molecular mechanisms

期刊名称：PLANTS-BASEL （ 2023影响因子：4.0； 五年影响因子：4.4 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 13 期

页码：

收录情况： SCI

摘要： The evolved resistance of Bromus japonicus Houtt. to ALS-inhibiting herbicides is well established. Previous studies have primarily focused on target-site resistance; however, non-target-site resistance has not been well characterized. This investigation demonstrated that ALS gene sequencing did not detect any previously known resistance mutations in a mesosulfuron-methyl-resistant (MR) population, and notably, treatment with the P450 monooxygenase (P450) inhibitor malathion markedly heightened susceptibility to mesosulfuron-methyl. Utilizing UPLC-MS/MS analysis confirmed elevated mesosulfuron-methyl metabolism in MR plants. The integration of Isoform Sequencing (Iso-Seq) and RNA Sequencing (RNA-Seq) facilitated the identification of candidate genes associated with non-target sites in a subpopulation with two generations of herbicide selection. Through qRT-PCR analysis, 21 differentially expressed genes were characterized, and among these, 10 genes (comprising three P450s, two glutathione S-transferases, one glycosyltransferase, two ATP-binding cassette transporters, one oxidase, and one hydrolase) exhibited constitutive upregulation in resistant plants. Our findings substantiated that increased herbicide metabolism is a driving force behind mesosulfuron-methyl resistance in this B. japonicus population.

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