Paralogous FgIDO genes with differential roles in tryptophan catabolism, fungal development and virulence in Fusarium graminearum

文献类型： 外文期刊

第一作者： Liu, Xin

作者： Liu, Xin;Wang, Liwen;Fang, Xin;Wang, Gang;Chen, Wenhua;Shi, Jianrong;Xu, Jianhong;Liu, Xin;Choera, Tsokyi;Keller, Nancy P.;Liu, Xin;Wang, Liwen;Fang, Xin;Wang, Gang;Shi, Jianrong;Xu, Jianhong;Lee, Yin-Won;Mohamed, Sherif Ramzy;Dawood, Dawood H.

作者机构：

关键词： Tryptophan catabolism; Indoleamine 2; 3-dioxygenases; Kynurenine pathway; Paralogous IDO genes; Fusarium graminearum; Virulence; Deoxynivalanol

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.7； 五年影响因子：7.1 ）

ISSN： 0944-5013

年卷期： 2023 年 272 卷

页码：

收录情况： SCI

摘要： Indoleamine 2,3-dioxygenase (Ido) is a tryptophan-degrading enzyme that is widely distributed across species. Ido catalyzes the first step of tryptophan (TRP) degradation and drives the de novo synthesis of nicotinamide adenine dinucleotide (NAD+) coenzymes via the kynurenine (KYN) pathway. The budding yeast Saccharomyces cerevisiae possesses a single IDO gene (BNA2) that is responsible for NAD+ synthesis, whereas a number of fungal species contain multiple IDO genes. However, the biological roles of IDO paralogs in plant pathogens remain unclear. In the current study, we identified three FgIDOs from the wheat head blight fungus Fusarium grami-nearum. FgIDOA/B/C expression was significantly induced upon TRP treatment. Targeted disruption of FgIDOA and/or FgIDOB caused different levels of NAD+ auxotrophy, thus resulting in pleotropic phenotypic defects. Loss of FgIDOA resulted in abnormal conidial morphology, reduced mycelial growth, decreased virulence in wheat heads and reduced deoxynivalenol accumulation. Exogenous addition of KYN or various intermediates involved in the KYN pathway rescued auxotrophy of the mutants. Metabolomics analysis revealed shifts toward alternative TRP degradation pathways to melatonin and indole derivatives in mutants lacking FgIDOB. Upregulation of partner genes in auxotrophic mutants and the capacity to rescue the auxotroph by overexpressing a partner gene indicated functional complementation among FgIDOA/B/C. Taken together, the results of this study provide insights into differential roles in paralogous FgIDOs and how fungal TRP catabolism modulates fungal devel-opment and virulence.

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