Host Responses to Live-Attenuated ASFV (HLJ/18-7GD)

文献类型： 外文期刊

第一作者： Fan, Yuqin

作者： Fan, Yuqin;Chen, Weiye;Jiang, Chenggang;Zhang, Xianfeng;Sun, Ying;Liu, Renqiang;Wang, Jingfei;Yang, Decheng;Zhao, Dongming;Bu, Zhigao;He, Xijun

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关键词： HLJ; 18-7GD strain; ASF; antibody; cytokine; T cell

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 9 期

页码：

收录情况： SCI

摘要： African swine fever (ASF) is a highly contagious and fatal disease caused by the African swine fever virus. Recently, the multigene family and CD2v gene-deleted ASF vaccine candidate HLJ/18-7GD was found to be safe and effective in laboratory and clinical trials. However, the immune-protective mechanisms underlying the effects of HLJ/18-7GD remain unclear. We assessed samples from pigs immunized with a single dose of 10(6) TCID50 HLJ/18-7GD. We found that pigs immunized with HLJ/18-7GD showed high levels of specific antibodies. T lymphocyte subsets (helper T cells (Th); cytotoxic T lymphocytes (CTL); double-positive T cells (DP-T cells)) were temporarily increased in peripheral blood mononuclear cells (PBMCs) after HLJ/18-7GD immunization. Once the HLJ/18-7GD-immunized pigs had been challenged with virulent HLJ/18, the percentage of Th, CTL, and DP-T cells increased significantly. PBMCs extracted from the pigs induced higher levels of CD8(+) T cells after infection with the HLJ/18 strain in vitro. The levels of GM-CSF, IFN-gamma, and TNF-alpha were upregulated at 7 days post-inoculation; this finding was contrary to the results obtained after HLJ/18 or HLJ/18 Delta CD2v infection. The immune protection from HLJ/18-7GD resulted from many synergies, which could provide a theoretical basis for HLJ/18-7GD as a safe and effective ASF vaccine.

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