Development of AIE fluorescent probe for real-time tracking GSH fluctuations in plants under heavy metal stress

文献类型： 外文期刊

第一作者： Zhang, Shiyi

作者： Zhang, Shiyi;Wang, Haiyang;Luo, Fei;Jiang, Lin;Liu, Luyao;Chen, Zizhen;Lai, Miao;Yang, Xiaopeng;Ji, Xiaoming;Yang, Yaqin;Zhang, Di

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关键词： GSH; Plant; Heavy metal stress; AIE; Fluorescent probe

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.7； 五年影响因子：7.4 ）

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年卷期： 2025 年 426 卷

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收录情况： SCI

摘要： Glutathione (GSH) is a crucial biological thiol involved in the ascorbate-glutathione cycle and phytochelatin synthesis in plants response to heavy metal stress. Traditional methods for detecting GSH often lack the capability for rapid, in situ analysis without damaging plant tissues. There is an urgent need to develop an effective method for real-time tracking of GSH fluctuations. Herein, we developed a triphenylamine-based aggregationinduced emission (AIE) fluorescent probe, TPA-NBD, for the detection of GSH. The probe features a rapid response time, low detection limit, and high selectivity for the detection of GSH. With AIE characteristics and low biotoxicity, TPA-NBD can effectively track exogenous GSH in plant cells and tissues. Additionally, TPA-NBD successfully monitored endogenous GSH fluctuations in Nicotiana tabacum L. seedlings subjected to Cr and Pbinduced heavy metal stress. Together, our studies demonstrate that TPA-NBD, as a novel tool for GSH detection in plants, offers reliable performance and significant potential for biological applications.

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