Neonatal Fc receptor participates in endocytosis of Fc fusion protein in vivo and in vitro

文献类型： 外文期刊

第一作者： Zhou, Yaping

作者： Zhou, Yaping;Zhao, Hongmei;Hao, Yongqing;Wang, Yanfang;Guo, Ting

作者机构：

关键词： FcRn; Fc fusion protein; Mucosal immunity; Endocytosis transport; Neonatal

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：1.4； 五年影响因子：1.6 ）

ISSN： 0165-2427

年卷期： 2025 年 283 卷

页码：

收录情况： SCI

摘要： The neonatal Fc receptor (FcRn) binds to IgG CH2 and CH3 domains (the Fc segment), triggering transendocytosis. Therefore, FcRn transports biological agents across the mucosal barrier. Mucosal administration provides less stimulation to the body than other methods. However, whether FcRn is an effective carrier for antigens across bovine respiratory epithelial cells is unknown. Here, an antigen was fused with the Fc fragment and transferred through the mucosal barrier to antigen-presenting cells via active transport mediated by FcRn. We established a model of FcRn-mediated recombinant IgG Fc protein expression in bovine embryonic tracheal epithelial cells. Western blotting showed that SPA inhibited the relative transport amount of FcRn-mediated IgG Fc fusion protein. Fc fusion protein positively correlated with protein concentration and action time, with the maximum level reached at 1.4 mg/mL (protein concentration) and 18 h (action time). An FcRn-mediated transport model of the IgG Fc recombinant protein in guinea pig lungs was established, and the amount of protein transported at different time points was measured using immunohistochemistry. FcRn mediates vaccine antigen delivery through the mucosal barrier to activate immune cells in the lamina propria, laying a theoretical foundation for the clinical application of nasal mucosal immune vaccines.

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