Fine mapping of qTGW2b and qGL9, two minor QTL conferring grain size and weight in rice

文献类型： 外文期刊

第一作者： Shi, Huan

作者： Shi, Huan;Yun, Peng;Zhu, Yun;Wang, Lu;Li, Pingbo;Lou, Guangming;Xia, Duo;Zhang, Qinglu;Xiao, Jinghua;Li, Xianghua;He, Yuqing;Gao, Guanjun;Yun, Peng

作者机构：

关键词： Rice; RIL; Grain size-related traits; QTL; qTGW2b; qGL9

期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）

ISSN： 1380-3743

年卷期： 2022 年 42 卷 11 期

页码：

收录情况： SCI

摘要： Rice grain size is a key determinant of both grain yield and quality. In this study, we conducted QTL mapping on grain size using a recombinant inbred line (RIL) population derived from a cross between japonica variety Beilu130 (BL130) and indica variety Jin23B (J23B). A total of twenty-two QTL related to grain length (GL), grain width (GW), grain length-to-width ratio (LWR), grain thickness (GT), and thousand grain weight (TGW) were detected under two environments, and 14 of them were repeatedly detected. Two minor QTL, qTGW2b and qGL9, were validated and further delimited to regions of 631 kb and 272 kb, respectively. Parental sequence comparison of genes expressed in inflorescence in corresponding candidate regions identified frameshifts in the exons of LOC_Os02g38690 and LOC_Os02g38780, both of which encode protein phosphatase 2C-containing protein, and LOC_Os09g29930, which encodes a BIM2 protein. Scanning electron microscopy (SEM) analysis revealed that the increase of cell size rather than cell number caused the differences in grain size between NILs of qTGW2b and qGL9. Quantitative RT-PCR analysis showed that the expression levels of EXPA4, EXPA5, EXPA6, EXPB3, EXPB4, and EXPB7 were significantly different in both qTGW2b NILs and qGL9 NILs. Our results lay the foundation for the cloning of qTGW2b and qGL9, and provide genetic materials for the improvement of rice yield and quality.

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