Comparative Transcriptomic Analysis of Differentially Expressed Transcripts Associated with Flowering Time of Loquat (Eriobotya japonica Lindl.)

文献类型： 外文期刊

第一作者： An, Haishan

作者： An, Haishan;Jiang, Shuang;Zhang, Jiaying;Xu, Fangjie;Zhang, Xueying;An, Haishan;Jiang, Shuang;Zhang, Jiaying;Xu, Fangjie;Zhang, Xueying

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关键词： differentially expressed transcript; Eriobotya japonica Lindl; flowering time; transcriptomic analysis

期刊名称：HORTICULTURAE （ 影响因子：2.331； 五年影响因子：2.951 ）

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年卷期： 2021 年 7 卷 7 期

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收录情况： SCI

摘要： Flowering is an important phenophase of plant species, however, knowledge about the regulatory mechanism controlling flowering cues in loquat is limited. To identify candidate genes regulating flowering time in loquat, we used RNA-Seq technology to conduct a comparative transcriptome analysis of differentiating apical buds collected from the early-flowering variety 'Baiyu' and the late-flowering variety 'Huoju'. A total of 28,842 differentially expressed transcripts (DETs) were identified. Of these, 42 DETs controlled flowering time while 17 other DETs were associated with the ABA signaling pathway. Compared with those in 'Huoju', EjFT, EjFY, EjFLK, and EjCAL1-like were significantly upregulated in 'Baiyu'. Moreover, transcripts of the ABA 8 '-hydroxylases (EjABH2, EjABH4, and EjABH4-like2), the ABA receptors (EjPYL4/8), and the bZIP transcription factor EjABI5-like were upregulated in 'Baiyu' compared with 'Huoju'. Hence, they might regulate loquat flowering time. There was no significant difference between 'Baiyu' and 'Huoju' in terms of IAA content. However, the ABA content was about ten-fold higher in the apical buds of 'Baiyu' than in those of 'Huoju'. The ABA:IAA ratio sharply rose and attained a peak during bud differentiation. Thus, ABA is vital in regulating floral bud formation in loquat. The results of the present study help clarify gene transcription during loquat flowering.

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