High plasticity of ribosomal DNA organization in budding yeast

文献类型： 外文期刊

第一作者： Jiang, Shuangying

作者： Jiang, Shuangying;Cai, Zelin;Zeng, Cheng;Zhang, Jiaying;Yu, Wenfei;Su, Chenghao;Zhao, Shijun;Ma, Yingxin;Dai, Junbiao;Jiang, Shuangying;Cai, Zelin;Zeng, Cheng;Zhang, Jiaying;Yu, Wenfei;Su, Chenghao;Zhao, Shijun;Ma, Yingxin;Dai, Junbiao;Cai, Zelin;Yu, Wenfei;Zhao, Shijun;Chen, Ying;Wang, Yun;Chen, Ying;Shen, Yue;Wang, Yun;Shen, Yue;Zhang, Jiaying;Cai, Yizhi;Dai, Junbiao;Dai, Junbiao

作者机构：

期刊名称：CELL REPORTS （ 影响因子：8.8； 五年影响因子：9.9 ）

ISSN： 2211-1247

年卷期： 2024 年 43 卷 2 期

页码：

收录情况： SCI

摘要： In eukaryotic genomes, rDNA generally resides as a highly repetitive and dynamic structure, making it difficult to study. Here, a synthetic rDNA array on chromosome III in budding yeast was constructed to serve as the sole source of rRNA. Utilizing the loxPsym site within each rDNA repeat and the Cre recombinase, we were able to reduce the copy number to as few as eight copies. Additionally, we constructed strains with two or three rDNA arrays and found that the presence of multiple arrays did not affect the formation of a single nucleolus. Although alteration of the position and number of rDNA arrays did impact the three-dimensional genome structure, the additional rDNA arrays had no deleterious influence on cell growth or transcriptomes. Overall, this study sheds light on the high plasticity of rDNA organization and opens up opportunities for future rDNA engineering.

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