Autophagy plays an antiviral defence role against tomato spotted wilt orthotospovirus and is counteracted by viral effector NSs

文献类型： 外文期刊

第一作者： Zhang, Xingwang

作者： Zhang, Xingwang;Hong, Hao;Yan, Jiaoling;Yuan, Yulong;Feng, Mingfeng;Liu, Qinhai;Zhao, Yanxiao;Yang, Tongqing;Huang, Shen;Wang, Chunli;Zhao, Ruizhen;Zuo, Wenyu;Liu, Suyu;Ding, Zixuan;Tao, Xiaorong;Huang, Changjun;Zhang, Zhongkai;Kundu, Jiban Kumar;Kundu, Jiban Kumar

作者机构：

关键词： antiviral defence; autophagy; counterdefence; nonstructural protein NSs; nucleocapsid protein; TSWV

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1464-6722

年卷期： 2024 年 25 卷 10 期

页码：

收录情况： SCI

摘要： Autophagy, an intracellular degradation process, has emerged as a crucial innate immune response against various plant pathogens, including viruses. Tomato spotted wilt orthotospovirus (TSWV) is a highly destructive plant pathogen that infects over 1000 plant species and poses a significant threat to global food security. However, the role of autophagy in defence against the TSWV pathogen, and whether the virus counteracts this defence, remains unknown. In this study, we report that autophagy plays an important role in antiviral defence against TSWV infection; however, this autophagy-mediated defence is counteracted by the viral effector NSs. Transcriptome profiling revealed the up-regulation of autophagy-related genes (ATGs) upon TSWV infection. Blocking autophagy induction by chemical treatment or knockout/down of ATG5/ATG7 significantly enhanced TSWV accumulation. Notably, the TSWV nucleocapsid (N) protein, a major component of the viral replication unit, strongly induced autophagy. However, the TSWV nonstructural protein NSs was able to effectively suppress N-induced autophagy in a dose-dependent manner. Further investigation revealed that NSs inhibited ATG6-mediated autophagy induction. These findings provide new insights into the defence role of autophagy against TSWV, a representative segmented negative-strand RNA virus, as well as the tospoviral pathogen counterdefence mechanism.

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