Non-Targeted Metabolomics Analysis of γ-Aminobutyric Acid Enrichment in Germinated Maize Induced by Pulsed Light

文献类型： 外文期刊

第一作者： Zhang, Liangchen

作者： Zhang, Liangchen;Xu, Liwei;Xie, Mengxi;Yu, Miao;Liu, Xiaojing

作者机构：

关键词： non-targeted metabolomics; gamma-aminobutyric acid; maize; pulsed light; germination; phytohormones

期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）

ISSN：

年卷期： 2024 年 13 卷 17 期

页码：

收录情况： SCI

摘要： Pulsed light is an emerging technique in plant physiology recognized for its ability to enhance germination and accumulate gamma-aminobutyric acid in maize. Pulsed light involves exposing plants to brief, high-intensity bursts of light, which can enhance photosynthesis, improve growth, and increase resistance to environmental stresses. Despite its promising potential, the specific metabolic changes leading to gamma-aminobutyric acid enrichment in maize induced by pulsed light are not fully understood. This study addresses this gap by quantifying key nutrients and gamma-aminobutyric acid-related compounds during maize germination and investigating the underlying mechanisms using non-targeted metabolomics. Our findings indicate that pulsed light significantly promotes maize germination and accelerates the hydrolysis of proteins, sugars, and lipids. This acceleration is likely due to the activation of enzymes involved in these metabolic pathways. Additionally, pulsed light markedly increases the content of glutamic acid and the activity of glutamate decarboxylase, which are crucial for gamma-aminobutyric acid synthesis. Moreover, pulsed light significantly reduces the activity of gamma-aminobutyric transaminase, thereby inhibiting gamma-aminobutyric acid decomposition and resulting in a substantial increase in gamma-aminobutyric acid content, with a 27.20% increase observed in germinated maize following pulsed light treatment. Metabolomic analysis further revealed enrichment of metabolic pathways associated with gamma-aminobutyric acid, including amino acid metabolism, carbohydrate metabolism, plant hormone signal transduction, energy metabolism, pyrimidine metabolism, and ABC transporters. In conclusion, pulsed light is a robust and efficient method for producing sprouted maize with a high gamma-aminobutyric acid content. This technique provides a novel approach for developing sprouted cereal foods with enhanced nutritional profiles, leveraging the physiological benefits of gamma-aminobutyric acid, which include stress alleviation and potential health benefits for humans.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序