FIP-fve Stimulates Cell Proliferation and Enhances IL-2 Release by Activating MAP2K3/p38 alpha (MAPK14) Signaling Pathway in Jurkat E6-1 Cells

文献类型： 外文期刊

第一作者： Gu, Kefei

作者： Gu, Kefei;Wang, Tan;Wang, Tan;Zhao, Yueliang;Wang, Tan;Zhao, Yueliang;Peng, Liying

作者机构：

关键词： FIP-fve; immunomodulation mechanism; proteomics analysis; MAP2K3/p38 alpha; IL-2

期刊名称：FRONTIERS IN NUTRITION （ 影响因子：6.59； 五年影响因子：6.873 ）

ISSN： 2296-861X

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： FIP-fve, a fungal fruiting body protein from Flammulina velutipes, has potential immunomodulatory properties. Here, we investigated the immunomodulation mechanism of FIP-fve in Jurkat E6-1 cells by conducting a cell viability assay and IL-2 release assay. Kinase inhibitors experiment and proteomics analysis were also involved in the mechanism study. It was found that FIP-fve stimulated cell proliferation and enhanced IL-2 secretion in a dose-dependent manner in Jurkat E6-1 cells. Unbiased high-throughput proteomics analysis showed that 4 T cell immune activation markers, including ZAP-70, CD69, CD82, and KIF23, were upregulated in response to FIP-fve treatment. Further pathway analysis indicated that MAP2K3/p38 pathway-related proteins, including MAP2K, p38, ELK, AATF, FOS, and JUN-B, were unregulated. In addition, losmapimod (p38 inhibitor) and gossypetin (MAP2K3 inhibitor) inhibited FIP-fve enhanced cell proliferation and IL-2 release in Jurkat E6-1 cells. Our results demonstrate that FIP-fve stimulates cell proliferation and enhances IL-2 secretion through MAP2K3/p38 alpha activation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序