Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress

文献类型： 外文期刊

第一作者： Ren Hai

作者： Ren Hai;Wu Jianhua;Ren Hai;Li Jian;Li Jitao;Liu Ping;Liang Zhongxiu

作者机构：

关键词： Exopalaemon carinicauda;mitochondrial manganese superoxide dismutase (mMnSOD);cytosolic manganese superoxide dismutase (cMnSOD);cloning;expression

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.068； 五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2015 年 33 卷 3 期

页码：

收录情况： SCI

摘要： Superoxide dismutase (SOD) is one of the most important antioxidant defense enzymes, and is considered as the first line against oxidative stress. In this study, we cloned a mitochondrial manganese (Mn) SOD (mMnSOD) cDNA from the ridgetail white prawn Exopalaemon carinicauda by using rapid amplification of cDNA ends (RACE) methods. The full-length cDNA for mMnSOD was 1 014-bp long, containing a 5'-untranslated region (UTR) of 37-bp, a 3'-UTR of 321-bp with a poly (A) tail, and included a 657-bp open reading frame encoding a protein of 218 amino acids with a 16-amino-acid signal peptide. The protein had a calculated molecular weight of 23.87 kDa and a theoretical isoelectric point of 6.75. The mMnSOD sequence included two putative N-glycosylation sites (NHT and NLS), the MnSOD signature sequence (180)DVWEHAYY(187), and four putative Mn binding sites (H48, H96, D180, and H184). Sequence comparison showed that the mMnSOD deduced amino acid sequence of E. carinicauda shared 97%, 95%, 89%, 84%, 82%, 72%, and 69% identity with that of Macrobrachium rosenbergii, Macrobrachium nipponense, Fenneropeneaus chinensis, Callinectes sapidus, Perisesarma bidens, Danio rerio, and Homo sapiens, resectively. Quantitative real-time RT-PCR analysis showed that mMnSOD transcripts were present in all E. carinicauda tissues examined, with the highest levels in the hepatopancreas. During an ammonia stress treatment, the transcript levels of mMnSOD and cMnSOD were up-regulated at 12 h in hemocytes and at 24 h in the hepatopancreas. As the duration of the ammonia stress treatment extended to 72 h, the transcript levels of mMnSOD and cMnSOD significantly decreased both in hemocytes and hepatopancreas. These findings indicate that the SOD system is induced to respond to acute ammonia stress, and may be involved in environmental stress responses in E. carinicauda.

分类号：

  • 相关文献

[1]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[2]Cloning of a heat shock protein 90 (HSP90) gene and expression analysis in the ridgetail white prawn Exopalaemon carinicauda. Li, Jitao,Han, Junying,Chen, Ping,Chang, Zhigjang,He, Yuying,Liu, Ping,Wang, Qingyin,Li, Jian. 2012

[3]Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress. Ren Hai,Ren Hai,Li Jian,Li Jitao,Ying Yu,Ge Hongxing,Li Dongli,Yu Tianji. 2015

[4]Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei.

[5]cDNA cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Gao, Baoquan,Chen, Ping,Duan, Yafei.

[6]The roles of Na+/K+-ATPase alpha-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses. Li, Jitao,Ma, Peng,Liu, Ping,Chen, Ping,Li, Jian.

[7]Cloning and Expression Analysis of Eight Upland Cotton Pentatricopeptide Repeat Family Genes. Han, Zongfu,Kong, Fanjin,Deng, Yongsheng,Wang, Zongwen,Shen, Guifang,Wang, Jinghui,Duan, Bing,Li, Ruzhong,Qin, Yuxiang.

[8]Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum. Pan, Hongyu,Wei, Yi,Xin, Furong,Zhou, Mingguo,Zhang, Shihong.

[9]Molecular cloning and expression analysis of CmMlo1 in melon. Cheng, Hong,Kun, Weiping,Liu, Dongshun,Su, Yongquan,He, Qiwei.

[10]Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media. Wan, Xia,Chai, Baozhong,Liao, Yi,Ye, Tao,Shen, Ping,Chen, Xiangdong,Wan, Xia,Su, Ying.

[11]Molecular cloning and mRNA expression of M-phase phosphoprotein 6 gene in black tiger shrimp (Penaeus monodon). Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang,Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang.

[12]Molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab Portunus trituberculatus. Li, Jitao,Chen, Ping,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[13]Toxoplasma gondii: Bioinformatics analysis, cloning and expression of a novel protein TgIMP1. Bai, Yang,He, Shenyi,Zhao, Guanghui,Chen, Lin,Shi, Na,Zhou, Huaiyu,Cong, Hua,Zhao, Qunli,Bai, Yang,He, Shenyi,Zhao, Guanghui,Chen, Lin,Shi, Na,Zhu, Xing-Quan,Zhu, Xing-Quan.

[14]Characterization of Schistosoma japonicum estrogen-related receptor beta like 1 and immunogenicity analysis of the recombinant protein. Wu, Xiujuan,Zhao, Bin,Hong, Yang,Li, Xuezheng,Peng, Jinbiao,Zhang, Juan,Wang, Fei,Shi, Yaojun,Fu, Zhiqiang,Lin, Jiaojiao.

[15]Isolation, characterization, and expression analysis of CmMLO2 in muskmelon. Cheng, Hong,Kong, Weiping,Hou, Dong,Tao, Xinglin,Lv, Junfeng.

[16]Molecular cloning and characterization of porcine interferon-induced protein with tetratricopeptide repeats (IFIT) 5. Zhang, Jiao,Shao, Si-yu,Li, Li-zu,Yang, Xiu-qin,Liu, Di.

[17]Expression, purification and molecular characterization of a novel endoglucanase protein from Bacillus subtilis SB13. Guan, Xuefang,Chen, Penglian,Xu, Qingxian,Qian, Lei,Huang, Juqing,Lin, Bin.

[18]Molecular cloning, tissue expression, and analysis with genome DNA methylation of porcine LSD1 gene. Chai, Jin,Liu, Lina,Chen, Chen,Peng, Yongdong,Jin, Wei,Zheng, Rong,Jiang, Siwen,Chai, Jin,Liu, Lina,Chen, Chen,Peng, Yongdong,Jin, Wei,Zheng, Rong,Jiang, Siwen,Xiong, Qi,Peng, Jian.

[19]Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage. Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Zhang, Dianchang,Huang, Jianhua,Guo, Yihui. 2008

[20]Cloning, expression of a peroxiredoxin gene from Acinetobacter sp SM04 and characterization of its recombinant protein for zearalenone detoxification. Yu, Yuanshan,Wu, Hui,Yu, Yuanshan,Wu, Hui,Tang, Yuqian,Qiu, Liping. 2012

作者其他论文 更多>>

微信小程序