Exploring the Subcellular Localization of Monascus Pigments Biosynthases: Preliminary Unraveling of the Compartmentalization Mechanism

文献类型： 外文期刊

第一作者： Xiong, Fei

作者： Xiong, Fei;Wei, Jingyi;Shao, Yanchun;Chen, Fusheng;Zhou, Youxiang;Liu, Jiao

作者机构：

关键词： Monascus spp.; Monascus pigments; Monascus pigments biosynthetic proteins; subcellular localization; compartmentalization

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 6 期

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收录情况： SCI

摘要： Monascus pigments (MPs), a class of secondary metabolites produced by Monascus spp., can be classified into yellow, orange, and red MPs according to their differences in the wavelength of the maximum absorption. However, the biosynthetic sequence and cellular biosynthesis mechanism of different MPs components are still not yet completely clear in Monascus spp. In this study, the subcellular localization of five MPs synthases was investigated using fluorescent protein fusion expression. The results revealed that the proteins encoded by the MPs biosynthetic gene cluster were compartmentalized in various subcellular locations, including the mitochondrial polyketide synthase MrPigA, cytosolic enzymes consisting of the ketoreductase MrPigC, the oxidoreductase MrPigE, and the monooxygenase MrPigN, and the cell-wall-bound oxidoreductase MrPigF. Moreover, the correct localization of MrPigF to the cell wall was crucial for the synthesis of orange MPs. Lastly, we discussed the compartmentalized biosynthetic pathway of MPs. This study will not only be helpful in clarifying the biosynthetic sequence and biosynthesis mechanism of different MPs but also provides new insights into the cellular biosynthesis of secondary metabolites in filamentous fungi.

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