Green tea fermented by Ganoderma lucidum presented anti-obesity properties via enhanced thermogenesis in vitro and on C57BL/6J mice

文献类型： 外文期刊

第一作者： Liu, Xuzhou

作者： Liu, Xuzhou;Zeng, Hongzhe;Wen, Shuai;Wang, Chao;Huang, Jianan;Liu, Zhonghua;Liu, Xuzhou;Ju, Ying;Jiang, Mingguo;Liu, Xuzhou;Tian, Bingchuan

作者机构：

关键词： Fermented tea; Ganoderma lucidum; Network pharmacology; Thermogenesis; Obesity

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）

ISSN： 0963-9969

年卷期： 2025 年 207 卷

页码：

收录情况： SCI

摘要： High-fat diets contribute to obesity and metabolic disorders. Ganoderma lucidum is renowned for its abundant bioactive compounds and diverse pharmacological effects. Green tea fermented by G. lucidum (TFG) has been shown to enhance lipid-lowering activity in vitro significantly. Using UPLC-MS/MS and GC-MS/MS, we identified 78 active lipid-lowering compounds in TFG. We explored their potential targets and pathways through network pharmacology, validated by in vivo experiments. In a 4-week trial, 70 mice were randomly assigned to 7 groups: ND (normal diet), HFD (high-fat diet), PC-HFD (HFD with orlistat), NFT1 (HFD with 200 mg/kg/day non-fermented tea), NFT2 (HFD with 400 mg/kg/day NFT), TFG1 (HFD with 200 mg/kg/day TFG), and TFG2 (HFD with 400 mg/kg/day TFG). TFG treatment significantly reduced body weight, hepatic lipid droplets, and epididymal adipocyte size in mice compared to the HFD group. TFG also increased the abundance of lipidlowering bacteria, such as Lactococcus and Lachnospirales. Liver transcriptomic and fecal metabolomic analyses revealed that TFG reduced triglyceride (TG), diglyceride (DG), monoglyceride (MG), and free fatty acid (FFA) levels and differentially regulated key genes (Dpf3, Atp5k, ND3) involved in the thermogenesis pathway. RT-PCR confirmed that TFG upregulated the mRNA expressions of AMPK, UCP1, PGC1 alpha, and PPAR gamma in dorsal fat. In conclusion, TFG enhances thermogenesis via the AMPK-PGC1 alpha pathway and increases the abundance of lipidlowering bacteria, thereby reducing fat accumulation in mice. These findings offer insights into TFG's antiobesity mechanisms, providing a scientific basis for developing new weight loss methods or products.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序