Characterization of the Clostridium perfringens phage endolysin cpp-lys and its application on lettuce

文献类型： 外文期刊

第一作者： Zhao, Xiaonan

作者： Zhao, Xiaonan;Li, Lulu;Zhang, Qing;Li, Mengxuan;Hu, Ming;Luo, Yanbo;Xu, Xiaohui;Chen, Yibao;Liu, Yuqing;Zhao, Xiaonan;Li, Lulu;Zhang, Qing;Li, Mengxuan;Hu, Ming;Luo, Yanbo;Xu, Xiaohui;Chen, Yibao;Liu, Yuqing

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关键词： Clostridium perfringens; Endolysin; Lettuce; Biocontrol

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）

ISSN： 0168-1605

年卷期： 2023 年 405 卷

页码：

收录情况： SCI

摘要： Clostridium perfringens is an important foodborne pathogen that can have severe consequences, including mor-tality and economic losses. In this study, the gene encoding cpp-lys, an endolysin from the C. perfringens phage cpp has been cloned and overexpressed. The encoded protein was characterized, and then its efficacy in con-trolling C. perfringens on lettuce was evaluated. The endolysin cpp-lys presented lytic activity against seven strains of C. perfringens that produce different types of toxins. It maintained stability across a wide range of temperatures (4 & DEG;C - 50 & DEG;C), and demonstrated tolerance to varying pH levels (4-9). Storage of endolysin cpp-lys under room-temperature conditions (16 & DEG;C-25 & DEG;C) and cold-temperature conditions (4 & DEG;C,-20 & DEG;C, and-80 & DEG;C) for 30 days did not affect its lytic activity. However, the lytic activity of cpp-lys decreased by 40 % and 18 % after storage for 30 d at 42 & DEG;C and 37 & DEG;C, respectively. The endolysin cpp-lys did not display cytotoxic activity against normal eukaryotic cells. The bacterial viability on lettuce was significantly lower in the group treated with endolysin cpp-lys than in the PBS group, and >4-log of C. perfringens J1 were removed within 15 min. Cpp-lys plus Zn2+ inhibited the activity of cpp-lys. The EDTA-treated cpp-lys significantly reduced the number of bac-teria by up to 0.6-log CFU compared with the endolysin cpp-lys group. The findings of this study demonstrated that endolysin cpp-lys has potential applications in controlling C. perfringens in the food industry.

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