CRISPR-Cas9-mediated editing of GmARM improves resistance to multiple stresses in soybean

文献类型： 外文期刊

第一作者： Luo, Tingting

作者： Luo, Tingting;Ma, Chongxuan;Fan, Yuanhang;Qiu, Zhendong;Tian, Yusu;Shang, Yuzhuo;Liu, Chang;Cao, Qingqian;Peng, Yuhan;Zhang, Shuzhen;Liu, Shanshan;Song, Bo;Li, Ming;Song, Bo

作者机构：

关键词： Soybean; Stress resistance; GmARM; CRISPR/Cas9

期刊名称：PLANT SCIENCE （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0168-9452

年卷期： 2024 年 346 卷

页码：

收录情况： SCI

摘要： The growth and development of soybean plants can be affected by both abiotic and biotic stressors, such as saline-alkali stress and Phytophthora root rot. In this study, we identified a stress-related gene-GmARM-whose promoter contained several hormone-response and stress-regulatory elements, including ABRE, TCA element, STRE, and MBS. qRT-PCR analysis showed that the expression of GmARM was the highest in seeds at 55 days after flowering. Furthermore, this gene was upregulated after exposure to saline-alkali stress and Phytophthora root rot infection at the seedling stage. Thus, we generated GmARM mutants using the CRISPR-Cas9 system to understand the role of this gene in stress response. T3 plants showed significantly improved salt tolerance, alkali resistance, and disease resistance, with a significantly higher survival rate than the wildtype plants. Moreover, mutations in GmARM affected the expression of related stress-resistance genes, indicating that GmARM mutants achieved multiple stress tolerance. Therefore, this study provides a foundation for further exploration of the genes involved in resistance to multiple stresses in soybean that can be used for breeding multiple stressresistance soybean varieties.

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