Rab7/Retromer-based endolysosomal trafficking is essential for proper host invasion in rice blast

文献类型： 外文期刊

第一作者： Chen, Xin

作者： Chen, Xin;Lin, Lili;Fang, Wenqin;Wu, Congxian;Yang, Piao;Zhang, Jin;Abubakar, Yakubu Saddeeq;Lu, Guodong;Wang, Zonghua;Zheng, Wenhui;Chen, Xin;Fang, Wenqin;Yang, Piao;Zhang, Jin;Abubakar, Yakubu Saddeeq;Lu, Guodong;Zheng, Wenhui;Selvaraj, Poonguzhali;Yang, Fan;Naqvi, Naweed I.;Lin, Lili;Liu, Wende;Wu, Congxian;Wang, Zonghua

作者机构：

关键词： effector secretion; endolysosomal trafficking; Magnaporthe oryzae; Rab GTPase MoRab7; retromer; SNARE

期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）

ISSN： 0028-646X

年卷期： 2023 年 239 卷 4 期

页码：

收录情况： SCI

摘要： Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection. Here, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits to the host interface and plasma membrane. To perform such secretory/trafficking function, MoRab7 first recruits the retromer complex to the vacuolar membrane, enabling recognition of a family of SNARE proteins, including MoSnc1. Live-cell imaging confirmed a highly dynamic vesicular trafficking of the retromer complex component(s) and MoSnc1 toward and across the host interface or plasma mem-brane, and subsequent fusion with target membranes. Interestingly, disruption of the MoRab7/Retromer/MoSnc1-based endolysosomal cascade affects effector secretion and fun-gal pathogenicity. Taken together, we discovered an unconventional protein and membrane trafficking route starting from the fungal endolysosomes to the M. oryzae-rice interaction interface and dissect the role of MoRab7/Retromer/MoSnc1 sorting machinery in effector secretion during biotro-phy and invasive growth in rice blast fungus.

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