Transcriptome Analysis Reveals Candidate Genes Involved in Calcium Absorption of Rosa roxburghii Plants and their Effects on the Bioactive Substance Accumulation in Fruit

文献类型： 外文期刊

第一作者： Wang, Zhao

作者： Wang, Zhao;Lu, Min;An, Huaming;Wang, Zhao

作者机构：

关键词： Rosa roxburghii; Calcium; Uptake and transport; RNA-Seq; Fruit quality

期刊名称：JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION （ 影响因子：3.9； 五年影响因子：3.9 ）

ISSN： 0718-9508

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： The objective of this research was to explore the candidate genes involved in calcium absorption of R. roxburghii plants, as well as their relationship with the bioactive substance accumulation in the fruit. RNA-seq and qRT-PCR were used to analyze the transcriptomic profiles of the roots and leaves of plants during calcium absorption. Based on the correlation between gene expression and calcium absorption rate, ion channels and carrier proteins were selected to further verify their response to exogenous calcium supplement and functions on the accumulation of ascorbate (AsA), flavonoid, and triterpenoid in the fruit. Transcriptome and qRT-PCR analysis revealed that a total of 25 candidate genes from seven families have been found to be closely associated with Ca2+ absorption, namely, cyclic nucleotide-gated ion channel (CNGC), zinc transporter (ZTP), metal tolerance protein (MTP), ER-type Ca(2+)ATPase (ECA), auto-inhibited Ca(2+)ATPase (ACA), glutamate receptor (GLR), and natural resistance-associated macrophage protein (NRAMP). Among these, RrCNGC1/7/10/12 and RrMTP2 play a vital role in responding to changes in external calcium levels. Moreover, exogenous Ca2+ promoted the accumulation of bioactive compounds such as AsA, total flavonoid, and total triterpenoid in fruits. RrCNGC2/3/12, RrZTP1, and RrGLR1 significantly responded to exogenous Ca2+ supplement, of which RrCNGC12 positively correlating with the levels of the above three types of bioactive substances. Seven families of ion channels and carrier proteins were jointly involved in calcium absorption and homeostasis in R. roxburghii plants, and RrCNGC12 play a critical role in the calcium-mediated regulation of fruit quality formation.

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