The maize transcription factor CCT regulates drought tolerance by interacting with Fra a 1, E3 ligase WIPF2, and auxin response factor Aux/IAA8

文献类型： 外文期刊

第一作者： Zhang, Zhaoheng

作者： Zhang, Zhaoheng;Qu, Jiayue;Lu, Min;Zhao, Xinyu;Xu, Yang;Wang, Li;Liu, Zhongjia;Shi, Yingying;Liu, Chaotian;Nan, Zhangjie;Cao, Qingqin;Pan, Jinbao;Li, Xinrui;Sun, Qingpeng;Wang, Weixiang;Li, Yipu;Wang, Chao;Xu, Mingliang;Li, Yipu;Liu, Wende

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关键词： Auxin; auxin response gene; drought tolerance; plant growth; ubiquitination; ZmCCT

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2024 年 75 卷 1 期

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收录情况： SCI

摘要： Plants are commonly exposed to abiotic stressors, which can affect their growth, productivity, and quality. Previously, the maize transcription factor ZmCCT was shown to be involved in the photoperiod response, delayed flowering, and quantitative resistance to Gibberella stalk rot. In this study, we demonstrate that ZmCCT can regulate plant responses to drought. ZmCCT physically interacted with ZmFra a 1, ZmWIPF2, and ZmAux/IAA8, which localized to the cell membrane, cytoplasm, and nucleus, respectively, both in vitro and in vivo in a yeast two-hybrid screen in response to abiotic stress. Notably, ZmCCT recruits ZmWIPF2 to the nucleus, which has strong E3 self-ubiquitination activity dependent on its RING-H2 finger domain in vitro. When treated with higher indole-3-acetic acid/abscisic acid ratios, the height and root length of Y331-Delta TE maize plants increased. Y331-Delta TE plants exhibited increased responses to exogenously applied auxin or ABA compared to Y331 plants, indicating that ZmCCT may be a negative regulator of ABA signalling in maize. In vivo, ZmCCT promoted indole-3-acetic acid biosynthesis in ZmCCT-overexpressing Arabidopsis. RNA-sequencing and DNA affinity purification-sequencing analyses showed that ZmCCT can regulate the expression of ZmRD17, ZmAFP3, ZmPP2C, and ZmARR16 under drought. Our findings provide a detailed overview of the molecular mechanism controlling ZmCCT functions and highlight that ZmCCT has multiple roles in promoting abiotic stress tolerance.

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